Ribas Mateus Rocha, Rocha Gustavo, Dal Pizzol Juliana Lemos, Wolleck Victor Felipe, Oliveira Vinicius Pais E, de Assunção Izadora Borgmann Frizzo, Klos Tainá Bittencourt, Carreira Lucas Parra Cesar Nogueira, de Abreu Débora Rodrigues, Kanaan Vanessa Tavares, Meurer Rafael, Caetano Franciele, Antonelli Marzia, Sandri Sandro, Kolesnikovas Cristiane Kiyomi Miyaji, Capella Daniel Barboza, Brito Guilherme Renzo Rocha, Valgas Cleidson, Sincero Thaís Cristine Marques, Palmeiro Jussara Kasuko
Laboratório de Microbiologia Molecular Aplicada (MiMA), Departamento de Análises Clínicas, Centro de Ciências da Saúde, Universidade Federal de Santa Catarina, Florianópolis, Santa Catarina, Brazil.
Instituto Catarinense de Conservação da Fauna e Flora, Florianópolis, Santa Catarina, Brazil.
Ecohealth. 2025 Jul 29. doi: 10.1007/s10393-025-01740-y.
Antimicrobial resistance (AMR) poses a growing threat to One Health, with wild birds serving as sentinels and reservoirs for resistant pathogens. Despite Brazil's rich avian biodiversity, studies on AMR in wild bird populations are limited. This study investigated methicillin-resistant Staphylococcaceae in wild birds from Southern Brazil, analyzing molecular characteristics of isolates. Oropharyngeal swabs were collected from 248 birds in two protected areas and two wildlife rehabilitation centers over one year. The sample included resident, partially migratory, and migratory species from terrestrial and aquatic habitats. Using selective culture and molecular tests, methicillin-resistant isolates were identified in 2.8% of the birds. These isolates also exhibited multidrug resistance and were predominantly found in resident birds within protected areas and nearby, suggesting environmental circulation of resistance genes. This is the first report of methicillin-resistant S. haemolyticus with SCCmec type I in Brazilian wild birds. The detection of mecA-positive isolates human-impacted areas underscores the need for ongoing surveillance. By addressing a critical knowledge gap, this study highlights the importance of monitoring AMR dynamics in biodiversity-rich regions to mitigate the spread of resistant bacteria and safeguard both environmental and public health.
抗菌药物耐药性（AMR）对“同一健康”构成了日益严重的威胁，野生鸟类充当了耐药病原体的哨兵和宿主。尽管巴西拥有丰富的鸟类生物多样性，但对野生鸟类种群中AMR的研究却很有限。本研究调查了巴西南部野生鸟类中的耐甲氧西林葡萄球菌科，分析了分离株的分子特征。在一年的时间里，从两个保护区和两个野生动物康复中心的248只鸟类中采集了口咽拭子。样本包括来自陆地和水生生境的留鸟、部分候鸟和候鸟物种。通过选择性培养和分子检测，在2.8%的鸟类中鉴定出耐甲氧西林分离株。这些分离株还表现出多重耐药性，主要在保护区内及附近的留鸟中发现，这表明耐药基因在环境中传播。这是巴西野生鸟类中首次报告携带I型SCCmec的耐甲氧西林溶血葡萄球菌。在受人类影响地区检测到mecA阳性分离株，凸显了持续监测的必要性。通过填补关键的知识空白，本研究强调了监测生物多样性丰富地区AMR动态对于减轻耐药细菌传播以及保障环境和公众健康的重要性。
