Liu Huan, Shi Wen, Zheng Yanjun, Yang Song, Wang Yihui, Yang Maosen, Yang Zhitao, Mao Enqiang, Shang Hanbing, Chen Erzhen, Chen Ying
Department of Emergency, Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200025, China.
Department of Emergency and Critical Care Medicine, Ruijin Hospital Wuxi Branch, Shanghai Jiao Tong University School of Medicine, Wuxi, 214112, China.
Sci Data. 2025 Jul 29;12(1):1312. doi: 10.1038/s41597-025-05620-6.
Sepsis is a severe disease and induces skeletal muscle atrophy, which has a significant impact on patients. Moreover, the onset and severity of muscle atrophy can vary across different anatomical regions during sepsis. Our previous study demonstrated that ZBED6 knockout not only promotes skeletal muscle growth under physiological conditions, but also alleviates systemic muscle atrophy during sepsis. However, its region-specific effects on sepsis-induced muscle atrophy have not been analyzed. In this study, we performed RNA sequencing for 54 samples of skeletal muscle from nine different anatomical regions from male ZBED6 knockout and wild-type (WT) minipigs subjected to sepsis. We generated 364.63 Gb of high-quality bulk RNA sequencing data from the skeletal muscle samples (approximately 6.75 Gb per sample). This dataset provided insightful gene expression information to understand the role of ZBED6 in region-specific muscle atrophy during sepsis. In addition, this dataset can be used for heterogeneity analysis between skeletal muscle tissues from multiple species or female pigs.
脓毒症是一种严重疾病，可导致骨骼肌萎缩，这对患者有重大影响。此外，脓毒症期间不同解剖区域的肌肉萎缩发作和严重程度可能有所不同。我们之前的研究表明，敲除ZBED6不仅能在生理条件下促进骨骼肌生长，还能减轻脓毒症期间的全身性肌肉萎缩。然而，其对脓毒症诱导的肌肉萎缩的区域特异性影响尚未得到分析。在本研究中，我们对来自遭受脓毒症的雄性ZBED6基因敲除和野生型（WT）小型猪九个不同解剖区域的54个骨骼肌样本进行了RNA测序。我们从骨骼肌样本中生成了364.63 Gb的高质量大量RNA测序数据（每个样本约6.75 Gb）。该数据集提供了有洞察力的基因表达信息，以了解ZBED6在脓毒症期间区域特异性肌肉萎缩中的作用。此外，该数据集可用于多个物种或雌性猪骨骼肌组织之间的异质性分析。
