Wang Yihan, Zhu Ke, Wang Yadong, Wang Ying, Xu Jie, Li Yuan, Liu Fang, Long Jinzhao, Yang Haiyan
Department of Epidemiology, School of Public Health, Zhengzhou University, No. 100 of Science Avenue, Zhengzhou, 450001, China.
Department of Toxicology, Henan Center for Disease Control and Prevention, Zhengzhou, 450016, China.
Mol Biol Rep. 2025 Jul 29;52(1):766. doi: 10.1007/s11033-025-10861-0.
Klebsiella pneumoniae (Kp) is an important conditional pathogen that causes bacteremia, pneumonia, sepsis, urinary tract infections, and liver abscesses. The emergence of multidrug-resistant Kp, especially carbapenem-resistant Kp (CRKP), has become a major challenge to global public health. There is a need for early identification and diagnosis of Kp pathogens as well as precise treatment of Kp infections. The CRISPR-Cas (Clustered regularly interspaced short palindromic repeats and associated Cas proteins) system is an adaptive immune mechanism for bacteria. Studies have shown that the presence of the CRISPR-Cas system is negatively correlated with drug resistance in Kp strains, and CRISPR-Cas system-based technologies have been developed for the detection, gene editing, the development of therapeutic drugs and vaccines in Kp infections. However, no review has been published on this area; therefore, this review describes the role of the CRISPR-Cas system in the development of antibiotic resistance in Kp, the application of CRISPR-Cas system-based technology in detection and gene editing, and the therapeutic potential of CRISPR-Cas system in Kp infections.
肺炎克雷伯菌（Kp）是一种重要的条件致病菌，可引起菌血症、肺炎、败血症、尿路感染和肝脓肿。多重耐药Kp，尤其是耐碳青霉烯类Kp（CRKP）的出现，已成为全球公共卫生的一大挑战。需要对Kp病原体进行早期鉴定和诊断，并对Kp感染进行精准治疗。CRISPR-Cas（成簇规律间隔短回文重复序列及其相关Cas蛋白）系统是细菌的一种适应性免疫机制。研究表明，CRISPR-Cas系统的存在与Kp菌株的耐药性呈负相关，并且基于CRISPR-Cas系统的技术已被开发用于Kp感染的检测、基因编辑、治疗药物和疫苗的研发。然而，该领域尚未发表综述；因此，本综述描述了CRISPR-Cas系统在Kp抗生素耐药性发展中的作用、基于CRISPR-Cas系统的技术在检测和基因编辑中的应用，以及CRISPR-Cas系统在Kp感染中的治疗潜力。
