He Chunping, Lin Jinjing, Wu He, Zheng Jinlong, Zhang Yong, Zhang Yu, Li Zengping, Liang Yanqiong, Lu Ying, Yi Kexian, Wu Weihuai
Key Laboratory of Integrated Pest Management on Tropical Crops, Environment and Plant Protection Institute, Ministry of Agriculture and Rural Affairs, Chinese Academy of Tropical Agricultural Science, Haikou 571101, China.
College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China.
Microorganisms. 2025 Jul 5;13(7):1586. doi: 10.3390/microorganisms13071586.
Rubber tree ( Muell. Arg.) is a major tropical cash crop in southern China, with Hainan and Yunnan provinces being the main planting areas. In July 2023, bark cracking and gumming were observed on the trunks of mature rubber trees in Haikou City, Hainan Province, leading to xylem rot, which severely impacted the healthy growth of the rubber trees. The present study was conducted to confirm the pathogenicity of the patho-gen associated with stem gummosis disease, characterize it using morphological and mo-lecular tools, and devise field management strategies. Pathogenicity testing showed that this strain induced symptoms similar to those of natural outdoor infestation. Based on morphological study and molecular analyses of internal transcribed spacer (), transla-tion elongation factor 1 alpha (), and β-tubulin 2 () sequences, the causal agent was identified as . Field trials demonstrated that an inte-grated fungicide approach-combining trunk application of Bordeaux mixture with root irrigation using citric acid-copper 6.4% + chelated copper-ammonium 15% at both 0.1% and 0.2% concentration-effectively suppressed stem gummosis disease incidence in rub-ber trees. To the best of our knowledge, this is the first report of causing stem gummosis on rubber tree in China. The findings of this study can provide valuable infor-mation for the management strategies and understanding of this disease.
橡胶树（缪勒·阿格）是中国南方的一种主要热带经济作物，海南省和云南省是主要种植区。2023年7月，在海南省海口市成熟橡胶树的树干上观察到树皮开裂和流胶现象，导致木质部腐烂，严重影响了橡胶树的健康生长。本研究旨在确认与茎流胶病相关病原菌的致病性，利用形态学和分子工具对其进行特征描述，并制定田间管理策略。致病性测试表明，该菌株引起的症状与自然户外侵染的症状相似。基于对内部转录间隔区（ITS）、翻译延伸因子1α（TEF1α）和β-微管蛋白2（TUB2）序列的形态学研究和分子分析，确定病原菌为[具体病原菌名称未给出]。田间试验表明，一种综合杀菌剂方法——将波尔多液树干涂抹与使用浓度为0.1%和0.2%的6.4%柠檬酸铜+15%螯合铜铵进行根部灌溉相结合，有效地抑制了橡胶树茎流胶病的发病率。据我们所知，这是中国首次报道[具体病原菌名称未给出]引起橡胶树茎流胶病。本研究结果可为该病的管理策略和认识提供有价值的信息。
