Bolboli Zeinab, Tavakolian Behnaz, Mostowfizadeh-Ghalamfarsa Reza, Jafari Moslem, Cacciola Santa Olga
Department of Plant Protection, School of Agriculture, Shiraz University, Shiraz 7144113131, Iran.
Fig Research Station, Fars Agricultural and Natural Resources Research and Education Center, Agricultural Research, Education and Extension Organization (AREEO), Estahban 7451877802, Iran.
J Fungi (Basel). 2022 Jun 30;8(7):694. doi: 10.3390/jof8070694.
Stem cankers and twig dieback were the most serious disease of fig () and loquat () noticed in a survey of fruit tree orchards in the Fars Province, Iran. Isolates of were consistently recovered from symptomatic fig and loquat trees. Phylogenetic analyses of multiple nuclear loci, internal transcribed spacer regions (ITS) of rDNA, RNA polymerase II subunit 2 (), and translation elongation factor 1-α (), combined with morphological observations, revealed that isolates could be referred to a still unknown taxon, which was formally described as sp. nov. Phylogenetically, isolates from fig and loquat trees clustered in a well-supported monophyletic group within the clade of , closely related to . sp. nov. was characterized by the lack of stilbella-like asexual structure in both natural substrates and pure cultures and produced two morphologically distinct types of conidia, globose and cylindrical, formed on short and long simple phialides. In pathogenicity tests, sp. nov. induced stem cankers in both fig and loquat, wood discoloration in fig and twig dieback in loquat. Pathogenicity tests also showed that the potential host range of this novel pathogen includes other economically relevant horticultural trees.
在伊朗法尔斯省的果园调查中，茎干溃疡和小枝枯死是无花果（）和枇杷（）最严重的病害。从有症状的无花果和枇杷树上持续分离到（）菌株。对多个核基因座、核糖体DNA的内转录间隔区（ITS）、RNA聚合酶II亚基2（）和翻译延伸因子1-α（）进行系统发育分析，并结合形态学观察，结果表明这些分离株可归为一个仍未知的分类单元，该分类单元被正式描述为新种（）。在系统发育上，从无花果和枇杷树上分离的菌株聚集在（）进化枝中一个得到充分支持的单系类群内，与（）密切相关。新种（）的特征是在自然基质和纯培养物中均缺乏类似小梗体的无性结构，并产生两种形态不同的分生孢子，即球形和圆柱形，分别形成于短的和长的简单瓶梗上。在致病性试验中，新种（）在无花果和枇杷上均引起茎干溃疡，在无花果上导致木材变色，在枇杷上引起小枝枯死。致病性试验还表明，这种新病原体的潜在寄主范围包括其他具有经济价值的园艺树木。
