Chen Yao, Cai Haotian, Wu Xiaoyang, Wang Xibao, Shang Yongquan, Wei Qinguo, Sha Weilai, Qi Yan, Liu Shuli, Zhang Honghai
College of Life Sciences, Qufu Normal University, Qufu 273165, China.
Zhonghuan Shengda Environmental Technology Group (Qingyun) Co., Ltd., Dezhou 253000, China.
Microorganisms. 2025 Jul 8;13(7):1604. doi: 10.3390/microorganisms13071604.
Mink () is a commercially farmed animal of global importance. However, disease outbreaks during farming not only cause significant economic losses but also substantially increase the risk of zoonotic infections. The identification and characterization of pathogenic bacteria remain a major bottleneck restricting the development of healthy and sustainable mink farming. In this study, an LB medium was used to isolate a pale-white, rod-shaped, Gram-negative bacterial strain, Qf-1, from minks with pneumonia. Based on morphological characteristics, biochemical properties, 16S rRNA gene sequencing, and average nucleotide identity (ANI) analysis, strain Qf-1 was identified as Qf-1. Under laboratory conditions, Qf-1 induced typical pneumonia symptoms in Kunming mice. Furthermore, whole-genome sequencing of Qf-1 revealed its genome to be 4.77 Mb and to contain a single chromosome and one plasmid. The main virulence genes of Qf-1 were primarily associated with , and , functioning in motility, biofilm formation, colonization ability, and secretion systems. Our findings contribute to a better understanding of their pathogenic mechanisms, thereby laying a theoretical foundation for further investigation into the complex interactions between gut microbiota and the host.
水貂是一种具有全球重要性的商业化养殖动物。然而，养殖过程中的疾病爆发不仅会造成重大经济损失，还会大幅增加人畜共患病感染的风险。病原菌的鉴定和特征描述仍然是限制健康和可持续水貂养殖发展的主要瓶颈。在本研究中，使用LB培养基从患有肺炎的水貂中分离出一株灰白色、杆状、革兰氏阴性细菌菌株Qf-1。基于形态特征、生化特性、16S rRNA基因测序和平均核苷酸同一性（ANI）分析，菌株Qf-1被鉴定为Qf-1。在实验室条件下，Qf-1在昆明小鼠中诱发了典型的肺炎症状。此外，Qf-1的全基因组测序显示其基因组大小为4.77 Mb，包含一条染色体和一个质粒。Qf-1的主要毒力基因主要与运动性、生物膜形成、定植能力和分泌系统有关。我们的研究结果有助于更好地理解其致病机制，从而为进一步研究肠道微生物群与宿主之间的复杂相互作用奠定理论基础。
