Qahl Safa H, Almohaimeed Hailah M, Algaidi Sami A, Batawi Ashwaq H, Mohammedsaleh Zuhair M, Abd-Elhamid Tarek Hamdy, Almohammadi Nawal H, Ayuob Nasra N, Mahmoud Amany Refaat
Department of Biology, College of Science, University of Jeddah, P.O. Box 80327, Jeddah 21589, Saudi Arabia.
Department of Basic Science, College of Medicine, Princess Nourah bint Abdulrahman University, P.O. Box 84428, Riyadh 11671, Saudi Arabia.
Pharmaceuticals (Basel). 2025 Jul 17;18(7):1046. doi: 10.3390/ph18071046.
: Recently, increased attention has been given to pumpkin due to its proved nutritional components, which include antioxidant, antifatigue, and anti-inflammatory effects. The aim of the present work was to assess the impact of L. () on chronic unpredictable mild stress (CUMS)-induced changes in lymphoid organs through evaluating its effect on the histological structure of spleen, thymus gland, and lymph nodes compared to the antidepressant fluoxetine (FLU). : Fifty male albino rats equally distributed into five groups that included control, control + , CUMS-exposed, FLU-treated, and -treated groups were used in this study. Rats were exposed to CUMS for 4 weeks, and treatment (either with FLU or ) was started after 14 days of exposure. Behavior of the rats, serum corticosterone, oxidants/antioxidants profile, proinflammatory cytokines, and gene expression of glucocorticoid receptor (GR) and β-adrenergic receptor (β2-AR) were assessed after 28 days. Spleen, thymus gland, and lymph nodes were histopathologically assessed. : administration significantly reduced the CUMS-induced behavioural changes evident by the significant reduction in immobility time ( = 0.02) and corticosterone level ( < 0.001). Biochemically, reduced TNF-α and IL-6 ( < 0.001) and markedly alleviated the changes in oxidants/antioxidants in the serum and lymphoid organs compared to fluoxetine. significantly ( < 0.001) reduced CUMS-induced changes in GR and (β2-AR). Histopathologically, alleviated changes observed in the spleen, lymph nodes, and thymus gland. It significantly reduced the number of CD4, CD8, CD68, CD20, and caspase-3 immunopositive cells in the studied organs. : This study proved the potential efficacy of in alleviating depression-associated immunodysregulation either alone or in combination with antidepressant therapy.
最近，由于南瓜具有抗氧化、抗疲劳和抗炎等已被证实的营养成分，人们对它的关注度有所增加。本研究的目的是通过评估其对脾脏、胸腺和淋巴结组织结构的影响，来评估L. () 对慢性不可预测轻度应激（CUMS）诱导的淋巴器官变化的影响，并与抗抑郁药氟西汀（FLU）进行比较。：本研究使用了50只雄性白化大鼠，将它们平均分为五组，包括对照组、对照组 + 、CUMS暴露组、FLU治疗组和 - 治疗组。大鼠暴露于CUMS 4周，并在暴露14天后开始治疗（用FLU或 ）。28天后评估大鼠的行为、血清皮质酮、氧化剂/抗氧化剂谱、促炎细胞因子以及糖皮质激素受体（GR）和β-肾上腺素能受体（β2-AR）的基因表达。对脾脏、胸腺和淋巴结进行组织病理学评估。：给药显著减少了CUMS诱导的行为变化，不动时间显著减少（ = 0.02），皮质酮水平降低（ < 0.001）。生化方面，与氟西汀相比， 降低了TNF-α和IL-6（ < 0.001），并显著缓解了血清和淋巴器官中氧化剂/抗氧化剂的变化。 显著（ < 0.001）减少了CUMS诱导的GR和（β2-AR）的变化。组织病理学上， 缓解了在脾脏、淋巴结和胸腺中观察到的变化。它显著减少了研究器官中CD4、CD8、CD68、CD20和caspase-3免疫阳性细胞的数量。：本研究证明了 单独或与抗抑郁治疗联合使用在减轻抑郁症相关免疫失调方面的潜在疗效。
