InstitutoMultidisciplinario de Biología Vegetal (IMBIV), CONICET and Departamento de Ciencias Farmacéuticas, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, X5000HUA Córdoba, Argentina.
Unidad de Investigación y Desarrollo en Tecnología Farmacéutica (UNITEFA), CONICET and Departamento de Ciencias Farmacéuticas, Facultad de Ciencias Químicas, Universidad Nacional de Córdoba, X5000HUA Córdoba, Argentina.
J Glob Antimicrob Resist. 2020 Sep;22:257-262. doi: 10.1016/j.jgar.2020.02.032. Epub 2020 Mar 10.
Methicillin-resistant Staphylococcus aureus (MRSA) strains are some of the most widespread pathogens with multi-resistant to antimicrobial agents (AA). AA provoke several changes inside bacteria, which cannot be solely explained by the main mechanisms of action reported.
The role of oxidative stress in bacteria exposed to bacteriostatic AA has not been widely studied; hence, the aim of our work was to investigate the effect of linezolid (LZD) on S. aureus strains.
Oxidative stress markers, such as superoxide dismutase (SOD) enzyme activity, the global antioxidant response, advanced oxidation protein products (AOPP) and basal levels of glutathione in 28 clinical and 2 reference strains were measured.
We identified 10 of 30 strains showing a slight increase in reactive species under LZD treatment with respect to the untreated control (between 22% and 56%). Higher generation was detected in clinical strains compared with the reference strains; however, the impact on the antioxidant response was not significant, and the oxidized protein levels were almost undetectable. The strains exposed to this oxazolidinone did not suffer acute oxidative stress. This is the first work reporting the behaviour of clinical and reference strains of S. aureus exposed to LZD, showing negligible oxidative stress.
耐甲氧西林金黄色葡萄球菌（MRSA）菌株是具有多种抗微生物药物（AA）耐药性的最广泛传播的病原体之一。AA 会在细菌内部引发多种变化，这些变化不能仅用已报道的主要作用机制来解释。
细菌抑菌 AA 暴露下的氧化应激作用尚未得到广泛研究；因此，我们的工作旨在研究利奈唑胺（LZD）对金黄色葡萄球菌菌株的影响。
测量了 28 株临床株和 2 株对照株的氧化应激标志物，如超氧化物歧化酶（SOD）酶活性、整体抗氧化反应、高级氧化蛋白产物（AOPP）和谷胱甘肽基础水平。
我们在未经处理的对照组中发现，在 LZD 处理下，30 株菌株中有 10 株的活性物质略有增加（增加幅度在 22%到 56%之间）。与对照株相比，临床株中检测到更高水平的活性物质；然而，对抗氧化反应的影响并不显著，氧化蛋白水平几乎检测不到。暴露于这种恶唑烷酮的菌株没有遭受急性氧化应激。这是首次报道利奈唑胺暴露的临床和对照金黄色葡萄球菌菌株的行为，表明氧化应激可忽略不计。
