Zhan Yujia, Zhao Xueshan, He Changyuan, Bi Siwei, Liu Ruiqi, Gu Jun, Yan Bin
Department of Cardiovascular Surgery, West China Hospital, Sichuan University, Chengdu 610000, China.
National Engineering Laboratory for Clean Technology of Leather Manufacture, College of Biomass Science and Engineering, Sichuan University, Chengdu 610000, China.
Polymers (Basel). 2025 Jul 12;17(14):1925. doi: 10.3390/polym17141925.
Postoperative adhesions are a prevalent complication following abdominal surgeries, often leading to significant clinical challenges. This study introduces an innovative solution utilizing a polyethylene glycol (PEG)-based triblock copolymer to form an injectable, self-healing hydrogel aimed at preventing these adhesions. The hydrogel, formulated with temperature-responsive and self-healing properties through the incorporation of poly (N-isopropyl acrylamide) (PNIPAM) and anion-pi interactions, was synthesized using reversible addition-fragmentation chain transfer (RAFT) polymerization. The hydrogel's physical properties, biocompatibility, hemostatic effect, and anti-adhesive capabilities were rigorously tested through in vitro and in vivo experiments involving rat models. It demonstrated excellent biocompatibility, effective tissue adhesion, and robust hemostatic properties. Most notably, it exhibited significant anti-adhesive effects in a rat abdominal wall-cecum model, reducing adhesion formation effectively compared to controls. The PEG-based injectable hydrogel presents a promising approach for postoperative adhesion prevention. Its ability to gel in situ triggered by body heat, coupled with its self-healing properties, provides a substantial advantage in clinical settings, indicating its potential utility as a novel anti-adhesion material.
术后粘连是腹部手术后常见的并发症，常常带来重大的临床挑战。本研究引入了一种创新解决方案，利用基于聚乙二醇（PEG）的三嵌段共聚物形成一种可注射的、自愈合水凝胶，旨在预防这些粘连。通过可逆加成-断裂链转移（RAFT）聚合反应合成了这种水凝胶，通过引入聚（N-异丙基丙烯酰胺）（PNIPAM）和阴离子-π相互作用使其具有温度响应和自愈合特性。通过涉及大鼠模型的体外和体内实验，对水凝胶的物理性质、生物相容性、止血效果和抗粘连能力进行了严格测试。它表现出优异的生物相容性、有效的组织粘附性和强大的止血性能。最值得注意的是，在大鼠腹壁-盲肠模型中它表现出显著的抗粘连效果，与对照组相比能有效减少粘连形成。基于PEG的可注射水凝胶为术后粘连预防提供了一种有前景的方法。它能够在体温触发下原位凝胶化，再加上其自愈合特性，在临床环境中具有显著优势，表明其作为一种新型抗粘连材料的潜在效用。
