Huang Yanyan, Liang Weiting, Lu Yunhui, Xiong Jie, Liu Dongmei, Jia Xiangze
Guangdong Provincial Key Laboratory of Intelligent Food Manufacturing, College of Food Science and Engineering, Foshan University, Foshan 528225, China.
School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
Nutrients. 2025 Jul 9;17(14):2265. doi: 10.3390/nu17142265.
This study investigated the properties of a neutral exopolysaccharide (EPS-LP1) with an average molecular weight of 55,637 Da, isolated from DMDL 9010 (LP9010).
The composition of EPS-LP1 includes galactose (Gal), glucose (Glu) and mannose (Man) in a molar ratio of 5.35:86.25:8.40. Notably, EPS-LP1 exhibits a smooth and rod-like surface along with thermal stability. Methylation combined with nuclear magnetic resonance analysis revealed that EPS-LP1 structured as t-Gal(1→, →6)-Glc(1→, 4)-Glc(1→ and →4,6)-Gal(1→), with relative molar ratio of 1.016:9.874:4.355:78.693:6.062, respectively. In the concentration range of 50 to 400 mg/mL, we observed the absence of cytotoxic effects from EPS-LP1 on RAW264.7 cells. Furthermore, EPS-LP1 demonstrated protective effects on RAW264.7 cells against oxidative damage by reducing the production of reactive oxygen species (ROS), malondialdehyde (MDA), and lactate dehydrogenase (LDH) release. Conversely, an increase in superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), and concentrations of glutathione (GSH) was observed. Immunoreactivity assays indicated that EPS-LP1 can effectively reduce the production of nitric oxide (NO) and inhibit the secretion of tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6). Additionally, it inhibited the activation of the mitogen-activated protein kinase (MAPK)/nuclear factor-kappa B gene binding (NF-kB) signaling pathway.
This research provides a foundation basis for further investigations into the neutral exopolysaccharide derived from LP9010.
本研究调查了从DMDL 9010（LP9010）中分离出的平均分子量为55,637 Da的中性胞外多糖（EPS-LP1）的性质。
EPS-LP1的组成包括半乳糖（Gal）、葡萄糖（Glu）和甘露糖（Man），摩尔比为5.35:86.25:8.40。值得注意的是，EPS-LP1呈现出光滑的棒状表面并具有热稳定性。甲基化结合核磁共振分析表明，EPS-LP1的结构为t-Gal(1→, →6)-Glc(1→, 4)-Glc(1→和→4,6)-Gal(1→)，相对摩尔比分别为1.016:9.874:4.355:78.693:6.062。在50至400 mg/mL的浓度范围内，我们观察到EPS-LP1对RAW264.7细胞没有细胞毒性作用。此外，EPS-LP1通过减少活性氧（ROS）、丙二醛（MDA）的产生以及乳酸脱氢酶（LDH）的释放，对RAW264.7细胞的氧化损伤具有保护作用。相反，观察到超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、谷胱甘肽过氧化物酶（GSH-Px）以及谷胱甘肽（GSH）浓度增加。免疫反应性测定表明，EPS-LP1可以有效减少一氧化氮（NO）的产生，并抑制肿瘤坏死因子-α（TNF-α）和白细胞介素-6（IL-6）的分泌。此外，它还抑制了丝裂原活化蛋白激酶（MAPK）/核因子-κB基因结合（NF-κB）信号通路的激活。
本研究为进一步研究源自LP9010的中性胞外多糖提供了基础依据。
