State Key Laboratory of Food Nutrition & Safety, Tianjin University of Science and Technology, Tianjin, PR China; Key Laboratory of Industrial Fermentation Microbiology, (Ministry of Education), Tianjin University of Science and Technology, Tianjin, PR China.
Tianjin Key Laboratory of Epigenetics for Organ Development in Preterm Infants, Tianjin, PR China.
Carbohydr Polym. 2022 Jul 15;288:119366. doi: 10.1016/j.carbpol.2022.119366. Epub 2022 Mar 21.
Exopolysaccharide (EPS-1) was isolated from Aureobasidium pullulans CGMCC 23063 and purified with DEAE 650M and Sephadex G-100 column chromatography. The structural characteristics and immunomodulatory activity of EPS-1 were investigated. The results showed EPS-1 (294.9 KDa) was β-glucan consisting of the backbone (1→3)-linked β-Glcp and β-(1,6)-branches by Ion chromatography, gas chromatography-mass spectrometry, and 1D/2D NMR spectroscopy. The SEM and AFM showed that EPS-1 composed spheres joined by triple-helix conformation into chains and circles. In vitro cell experiments, EPS-1 displayed immunological activity on the RAW264.7 cells. After being treated with EPS-1 (400 μg/mL), the concentration of NO, TNF-α, and IL-6 could reach 17.05 μM, 448.46 pg/mL, and 15.18 pg/mL, respectively. In addition, the cytokines mRNA levels also demonstrated the above results at the molecular level. The western-blot results demonstrated that the expression of phosphorylated p65 and p-erk associated with cytokine release, was increased with the dose of EPS-1.
从出芽短梗霉 CGMCC 23063 中分离得到胞外多糖（EPS-1），并通过 DEAE 650M 和 Sephadex G-100 柱层析进行纯化。研究了 EPS-1 的结构特征和免疫调节活性。结果表明，EPS-1（294.9 kDa）是一种β-葡聚糖，由离子色谱、气相色谱-质谱联用和 1D/2D NMR 光谱分析得出，其主链由（1→3）连接的β-Glcp 和β-（1,6）-支链组成。SEM 和 AFM 显示，EPS-1 由三螺旋构象连接的球体组成链和环。体外细胞实验表明，EPS-1 对 RAW264.7 细胞具有免疫活性。经 EPS-1（400 μg/mL）处理后，NO、TNF-α 和 IL-6 的浓度分别达到 17.05 μM、448.46 pg/mL 和 15.18 pg/mL。此外，细胞因子 mRNA 水平也在分子水平上证明了上述结果。Western-blot 结果表明，与细胞因子释放相关的磷酸化 p65 和 p-erk 的表达随 EPS-1 剂量的增加而增加。
