Tantipat Supreeya, Trisuwan Kongkiat, Kunnaja Phraepakaporn, Sireeratawong Seewaboon, Natakankitkul Surapol, Imiam Surasak, Chansakaow Sunee
Department of Pharmaceutical Sciences, Faculty of Pharmacy, Chiang Mai University, Chiang Mai 50200, Thailand.
Department of Chemistry, Faculty of Science, Chiang Mai University, Chiang Mai 50200, Thailand.
Pharmaceutics. 2025 Jun 30;17(7):864. doi: 10.3390/pharmaceutics17070864.
: Wannachawee recipe (WCR) has been listed in the Hospital Traditional Medicine Formulary and has been used as a Thai medicine to treat psoriasis in the Thai Traditional Medicine Clinic of Prapokklao Hospital since 2006. Previous reports have found that WCR demonstrates good results for the treatment of patients with psoriasis. Among 136 Thai psoriasis patients who received WCR, 92.80% responded well. Although WCR is effective, there is still a lack of scientific data, especially relating to the bioactive compound in WCR. Therefore, this study aims to evaluate the phytochemicals in WCR via bioassay-guided isolation. : In this study, the WCR was extracted via decoction with water, in a process based on traditional Thai medicine. The water extract was concentrated and dried using a spray dryer. The crude water extract was isolated using the partition technique with organic solvents, namely petroleum ether and ethyl acetate. These fractions were then separated and tested for anti-inflammatory activity using the bioassay-guided fractionation method. : Two particular types of pro-inflammatory cytokines are involved in inflammation and are among the factors that cause psoriasis-TNF- and IL-6. Thus, we evaluated the isolated samples in terms of anti-inflammatory activity. The isolation resulted in two pure compounds--coumaryl aldehyde and --coumaryl alcohol. In the efficacy test of the isolated compounds, compared to the standard indomethacin at the same concentration of 12.5 ug/mL, --coumaryl alcohol was found to have the best efficacy, inhibiting TNF- by 29.28% and IL-6 by 36.75%, with the standard compound showing inhibitions rates of 15.80% for TNF- and 27.44% for IL-6. : This study is the first report to identify the bioactive compound of WCR as --coumaryl alcohol or 4-hydroxycinnamyl alcohol.
万纳查维配方（WCR）已被列入医院传统医学处方集，自2006年以来一直在普拉波克拉奥医院的泰国传统医学诊所作为泰国药物用于治疗银屑病。先前的报告发现，WCR对银屑病患者的治疗显示出良好效果。在136名接受WCR治疗的泰国银屑病患者中，92.80%反应良好。尽管WCR有效，但仍缺乏科学数据，尤其是与WCR中的生物活性化合物相关的数据。因此，本研究旨在通过生物测定引导分离来评估WCR中的植物化学物质。
在本研究中，WCR采用基于泰国传统医学的水煮法提取。水提取物经浓缩后用喷雾干燥器干燥。粗水提取物采用与有机溶剂（即石油醚和乙酸乙酯）的分配技术进行分离。然后使用生物测定引导分级分离法对这些馏分进行分离并测试其抗炎活性。
两种特定类型的促炎细胞因子参与炎症反应，是导致银屑病的因素之一——肿瘤坏死因子（TNF-）和白细胞介素-6（IL-6）。因此，我们从抗炎活性方面对分离出的样品进行了评估。分离得到了两种纯化合物——香豆醛和香豆醇。在分离化合物的功效测试中，与浓度为12.5μg/mL的标准吲哚美辛相比，香豆醇的功效最佳，对TNF-的抑制率为29.28%，对IL-6的抑制率为36.75%，标准化合物对TNF-的抑制率为15.80%，对IL-6的抑制率为27.44%。
本研究是首次报道将WCR的生物活性化合物鉴定为香豆醇或4-羟基肉桂醇。
