Stabrauskiene Jolita, Mazurkevičiūtė Agnė, Majiene Daiva, Balanaskiene Rima, Bernatoniene Jurga
Department of Drug Technology and Social Pharmacy, Faculty of Pharmacy, Medical Academy, Lithuanian University of Health Sciences, 44307 Kaunas, Lithuania.
Institute of Pharmaceutical Technologies, Faculty of Pharmacy, Medical Academy, Lithuanian University of Health Sciences, 44307 Kaunas, Lithuania.
Pharmaceutics. 2025 Jul 19;17(7):933. doi: 10.3390/pharmaceutics17070933.
Chronic inflammatory skin disorders, such as atopic dermatitis and psoriasis, require safe and effective topical treatments. This study aimed to develop and evaluate a novel anti-inflammatory emulsion enriched with menthol, capsaicin, amino acids (glycine, arginine, histidine), and boswellic acid. Three formulations were prepared: a control (E1), a partial (E2), and a comprehensive formulation (E3). Physicochemical analyses included texture profiling, rheological behavior, pH stability, moisture content, and particle size distribution. E3 demonstrated superior colloidal stability, optimal pH (5.75-6.25), and homogenous droplet size (<1 µm), indicating favorable dermal delivery potential. Ex vivo permeation studies revealed effective skin penetration of menthol and amino acids, with boswellic acid remaining primarily in the epidermis, suggesting localized action. Under oxidative stress conditions, E3 significantly improved fibroblast viability, indicating synergistic cytoprotective effects of combined active ingredients. While individual compounds showed limited or dose-dependent efficacy, their combination restored cell viability to near-control levels. These findings support the potential of this multi-component emulsion as a promising candidate for the topical management of inflammatory skin conditions.
慢性炎症性皮肤病，如特应性皮炎和银屑病，需要安全有效的局部治疗方法。本研究旨在开发和评估一种富含薄荷醇、辣椒素、氨基酸（甘氨酸、精氨酸、组氨酸）和乳香酸的新型抗炎乳液。制备了三种配方：对照配方（E1）、部分配方（E2）和综合配方（E3）。物理化学分析包括质地分析、流变行为、pH稳定性、水分含量和粒径分布。E3表现出优异的胶体稳定性、最佳pH值（5.75 - 6.25）和均匀的液滴尺寸（<1 µm），表明其具有良好的皮肤递送潜力。体外渗透研究表明薄荷醇和氨基酸能够有效渗透皮肤，而乳香酸主要保留在表皮层，提示其具有局部作用。在氧化应激条件下，E3显著提高了成纤维细胞的活力，表明组合活性成分具有协同细胞保护作用。虽然单一化合物显示出有限的或剂量依赖性的功效，但它们的组合将细胞活力恢复到接近对照水平。这些研究结果支持了这种多组分乳液作为炎症性皮肤病局部治疗的有前景候选药物的潜力。
