Souza Melissa P, de Castro Maria V L, Barbosa Gabriela A da C, Carvalho Sabrine G, Coelho Amanda M R M, Dias Rosane B, Soares Milena B P, Costa Emmanoel V, Bezerra Daniel P
Postgraduate Program in Chemistry, Institute of Exact Sciences, Federal University of Amazonas (UFAM), Manaus 69080-900, Amazonas, Brazil.
Department of Chemistry, Institute of Exact Sciences, Federal University of Amazonas (UFAM), Manaus 69080-900, Amazonas, Brazil.
Molecules. 2025 Jul 15;30(14):2971. doi: 10.3390/molecules30142971.
H. Rainer (Annonaceae) is a tree native to the Amazon rainforest. Its fruits are also suitable for human consumption in their natural state or are processed to make desserts. In this work, we characterized the chemical composition of the essential oil (EO) from the leaves of and evaluated its anti-liver-cancer potential via in vitro and in vivo approaches. Chemical composition analysis revealed β-elemene, ()-caryophyllene, germacrene D, and germacrene B as the main constituents. The EO had IC values ranging from 12.28 to 37.50 μg/mL for B16-F10 cells and MCF-7 cells, whereas an IC value of >50 μg/mL was found for noncancerous MRC-5 cells. DNA fragmentation, YO-PRO-1 staining, and loss of mitochondrial transmembrane potential were detected in EO-treated HepG2 cells, indicating the induction of apoptosis. Significant in vivo growth inhibition of 53.7% was observed in mice bearing HepG2 cell xenografts treated with EO at a dosage of 40 mg/kg. These data suggest that EO from leaves is a drug source for liver cancer.
H. Rainer（番荔枝科）是一种原产于亚马逊雨林的树木。其果实以天然状态也适合人类食用，或被加工制作甜点。在这项工作中，我们对来自[该植物名称未完整给出]叶子的精油（EO）的化学成分进行了表征，并通过体外和体内方法评估了其抗肝癌潜力。化学成分分析表明，β-榄香烯、（）-石竹烯、杜松烯D和杜松烯B是主要成分。该精油对B16-F10细胞和MCF-7细胞的IC值范围为12.28至37.50μg/mL，而对于非癌性MRC-5细胞，IC值>50μg/mL。在经精油处理的HepG2细胞中检测到DNA片段化、YO-PRO-1染色和线粒体跨膜电位丧失，表明诱导了细胞凋亡。在用40mg/kg剂量的精油处理的携带HepG2细胞异种移植瘤的小鼠中，观察到显著的体内生长抑制率为53.7%。这些数据表明，来自[该植物名称未完整给出]叶子的精油是肝癌的一种药物来源。
