Xu Jiao, Wang Qinghua, Yu Jiarong, Wang Yingli, Li Huicong, Li Lin, Bao Jingyue, Wang Zhiliang
China Animal Health and Epidemiology Center, Qingdao, 266000, China.
College of Veterinary Medicine, Qingdao Agricultural University, Qingdao, 266109, China.
Viruses. 2025 Jul 12;17(7):976. doi: 10.3390/v17070976.
Peste des petits ruminants virus (PPRV) has been classified into four lineages based on the nucleocapsid and fusion genes, with lineage IV strains being the most widely distributed. In Africa, recent epidemiological data revealed that PPRV lineage IV is increasingly displacing other lineages in prevalence, suggesting a competitive advantage in viral transmission and adaptability. Moreover, a lineage IV strain was the only confirmed strain in Europe and Asia. In this study, a one-step Taqman quantitative real-time reverse transcription polymerase chain reaction (RT-qPCR) assay for lineage IV PPRV was established by targeting the hemagglutinin (H) gene. The results indicated that this method could detect approximately six copies of PPRV RNA, indicating high sensitivity. No cross-reactions with related viruses or other lineages of PPRV were observed. The results of a repeatability test indicated that the coefficient of variation values were low in both the inter-assay and intra-assay experimental groups. Detection of field samples indicated that all positive samples could be detected successfully using the developed method. This RT-qPCR assay provides a valuable tool to facilitate targeted surveillance and rapid differential diagnosis in regions with active circulation of PPRV lineage IV, enabling timely epidemiological investigations and strain-specific identification.
小反刍兽疫病毒（PPRV）根据核衣壳基因和融合基因已被分为四个谱系，其中IV谱系毒株分布最为广泛。在非洲，最近的流行病学数据显示，PPRV IV谱系在流行率方面正越来越多地取代其他谱系，这表明其在病毒传播和适应性方面具有竞争优势。此外，IV谱系毒株是欧洲和亚洲唯一确诊的毒株。在本研究中，通过靶向血凝素（H）基因建立了一种用于检测IV谱系PPRV的一步法Taqman定量实时逆转录聚合酶链反应（RT-qPCR）检测方法。结果表明，该方法可检测到约6个拷贝的PPRV RNA，显示出高灵敏度。未观察到与相关病毒或PPRV其他谱系的交叉反应。重复性试验结果表明，批间和批内实验组的变异系数值均较低。对现场样本的检测表明，使用所建立的方法能够成功检测到所有阳性样本。这种RT-qPCR检测方法为在PPRV IV谱系活跃传播的地区开展针对性监测和快速鉴别诊断提供了一个有价值的工具，有助于及时进行流行病学调查和毒株特异性鉴定。
