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对从接种疫苗的母牛获得的经巴氏杀菌初乳池喂养的犊牛从出生到断奶期间初乳中牛病毒性腹泻病毒(BVDV)、牛疱疹病毒1型(BoHV-1)和牛呼吸道合胞病毒(BRSV)特异性血清抗体水平的现场监测

Field Monitoring of Colostral BVDV-, BoHV-1-, and BRSV-Specific Serum Antibody Levels in Dairy Calves from Birth to Weaning Fed with Pasteurized Colostrum Pools Obtained from Vaccinated Dams.

作者信息

Ataseven Veysel Soydal, Kaya Ufuk, Doğan Müge, Şengül Sultan, Turan Seda, Türkarslan Akbaba Fatma, Kocaer İsmail İlker

机构信息

Department of Virology, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, 31060 Hatay, Türkiye.

Department of Biostatistics, Faculty of Veterinary Medicine, Hatay Mustafa Kemal University, 31060 Hatay, Türkiye.

出版信息

Vaccines (Basel). 2025 Jun 29;13(7):709. doi: 10.3390/vaccines13070709.

Abstract

: This study aimed to determine the changes in BVDV (bovine viral diarrhea virus), BoHV-1 (bovine herpesvirus-1), and BRSV (bovine respiratory syncytial virus) antibody levels until weaning in calves who ingested colostrum from vaccinated dairy cattle. Additionally, it aimed to measure the antibody levels induced by the vaccine administered before and after socialization after weaning. : Exposure to respiratory viral and bacterial agents was monitored by PCR analysis using nasal swabs at regular intervals from birth to weaning (pre-colostral and after the 2nd, 7th, 15th, 25th, 35th, 45th, 55th, and 65th days). The levels of colostral BVDV, BoHV-1, and BRSV antibodies were monitored using an enzyme-linked immunosorbent assay (ELISA) at the same intervals from birth to weaning (pre-colostral and after the 2nd, 7th, 15th, 25th, 35th, 45th, 55th, and 65th days). : The highest level of maternal antibodies in the blood was detected on day 7. BoHV-1, BVDV, and BRSV antibody levels decreased steadily until weaning by 69.14%, 38%, and 53%, respectively. : Vaccination strategies should be planned by considering the presence of maternally derived antibodies and minimizing stress that may negatively affect vaccine titers, thus maximizing vaccine efficacy in calves.

摘要

本研究旨在确定摄入来自接种疫苗奶牛初乳的犊牛在断奶前牛病毒性腹泻病毒(BVDV)、牛疱疹病毒1型(BoHV - 1)和牛呼吸道合胞病毒(BRSV)抗体水平的变化。此外,其旨在测量断奶后社交前后接种疫苗所诱导的抗体水平。:从出生到断奶(初乳前以及第2、7、15、25、35、45、55和65天之后),定期使用鼻拭子通过PCR分析监测呼吸道病毒和细菌病原体的暴露情况。从出生到断奶(初乳前以及第2、7、15、25、35、45、55和65天之后),以相同间隔使用酶联免疫吸附测定(ELISA)监测初乳中BVDV、BoHV - 1和BRSV抗体水平。:血液中母体抗体的最高水平在第7天检测到。直到断奶时,BoHV - 1、BVDV和BRSV抗体水平分别稳步下降69.14%、38%和53%。:应通过考虑母源抗体的存在并尽量减少可能对疫苗效价产生负面影响的应激来规划疫苗接种策略,从而使犊牛的疫苗效力最大化。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/21ce/12298652/3e305e6684bc/vaccines-13-00709-g001.jpg

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