高效液相色谱法测定人体生物体液中的α-鹅膏毒肽和β-鹅膏毒肽
Determination of alpha-amanitin and beta-amanitin in human biological fluids by high-performance liquid chromatography.
作者信息
Jehl F, Gallion C, Birckel P, Jaeger A, Flesch F, Minck R
出版信息
Anal Biochem. 1985 Aug 15;149(1):35-42. doi: 10.1016/0003-2697(85)90474-9.
A high-performance liquid chromatographic assay of alpha-amanitin and beta-amanitin in human serum, urine, or stomach washings is described. Sample preparation involves a chemical step with deproteinization and organic solvent treatment, and a selective cleanup and concentration step on reversed-phase prepacked cartridges. Separations are performed on a reversed-phase analytical column under isocratic conditions with uv detection at 280 nm. The method allows the quantitation of alpha- and beta-amanitin separately with a detection limit of 10 ng/ml for both toxins.
本文描述了一种用于检测人血清、尿液或洗胃洗液中α-鹅膏毒肽和β-鹅膏毒肽的高效液相色谱法。样品制备包括一个脱蛋白和有机溶剂处理的化学步骤，以及一个在反相预填充柱上进行的选择性净化和浓缩步骤。分离在反相分析柱上于等度条件下进行，紫外检测波长为280nm。该方法能够分别定量α-鹅膏毒肽和β-鹅膏毒肽，两种毒素的检测限均为10ng/ml。
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