Xi Na, Liu Teng-Da, Zhang Yu-Zhou, Liu Kuo, Du Hua-Ying, Gu Pei-Wen, Yu Ze-Yang, Song Yu-Yang
School of Agriculture, Ningxia University, Yinchuan, Ningxia, China.
Ningxia Rural Science and Technology Development Center, Yinchuan, Ningxia, China.
Front Microbiol. 2025 Jul 15;16:1601945. doi: 10.3389/fmicb.2025.1601945. eCollection 2025.
is a kind of plant pathogenic fungus that is widely present in the soil. It can trigger a variety of plant diseases, such as wilt and root rot, which pose a serious threat to agricultural production.
In this study, a strain of was identified by morphology, physiology, biochemistry, and molecular biology and was named LK-1.
The antagonistic curve showed that the best antagonistic fermentation time was 48 h. The fermentation broth and bacterial suspension of LK-1 had sound antagonistic effects on the mycelium or spores of ; when the mycelium or spores of were treated with LK-1, they grew abnormally. Compared to fermentation broth, the antagonistic effect of sterile filtrate was lower. In addition, compared to the seedlings in the control group, the plant height, root length, aboveground fresh weight, underground fresh weight, aboveground dry weight, underground dry weight, and leaf area indexes of seedlings treated with LK-1 fermentation broth (1 × 10 cfu/mL) were all significantly higher. Using the one-way test, Plackett-Burman test, and Box-Behnken test, the fermentation medium and conditions for strain LK-1 were ultimately determined to be 0.5% glucose, 2% beef extract, 2% NaCl, and 0.5% yeast powder, with a fermentation time of 46 h, an inoculum size of 1%, a pH of 6.6, and a rotational speed of 170 rpm. Validation experiments showed that the bacterial inhibition rate under these conditions reached 62.5%, which was 1.43% higher than the theoretical value and 8.06% higher than the unoptimized value (54.44%).
LK-1 had sound preventive and control effects against many kinds of .
是一种广泛存在于土壤中的植物病原真菌。它能引发多种植物病害，如枯萎病和根腐病，对农业生产构成严重威胁。
本研究通过形态学、生理学、生物化学和分子生物学方法鉴定出一株，并将其命名为LK-1。
拮抗曲线表明最佳拮抗发酵时间为48小时。LK-1的发酵液和菌悬液对的菌丝体或孢子具有良好的拮抗作用；当用LK-1处理的菌丝体或孢子时，它们生长异常。与发酵液相比，无菌滤液的拮抗作用较低。此外，与对照组的幼苗相比，用LK-1发酵液（1×10 cfu/mL）处理的幼苗的株高、根长、地上鲜重、地下鲜重、地上干重、地下干重和叶面积指数均显著更高。通过单因素试验、Plackett-Burman试验和Box-Behnken试验，最终确定菌株LK-1的发酵培养基和条件为0.5%葡萄糖、2%牛肉膏、2%氯化钠和0.5%酵母粉，发酵时间为46小时，接种量为1%，pH值为6.6，转速为170 rpm。验证实验表明，在这些条件下抑菌率达到62.5%，比理论值高1.43%，比未优化值（54.44%）高8.06%。
LK-1对多种具有良好的防治效果。
