Ocmen Elvan, Erdost Hale Aksu, Yilmaz Osman, Bagriyanik Alper, Kiray Muge, Gokmen Necati
Department of Anesthesia, Dokuz Eylul University Hospital, Izmir 35330, Turkey.
Department of Anesthesia, Dokuz Eylul University School of Health Sciences, Izmir 35330, Turkey.
Asian Biomed (Res Rev News). 2025 Jul 28;19(3):141-146. doi: 10.2478/abm-2025-0017. eCollection 2025 Jun.
Anesthesia has been linked to neuroapoptosis and prolonged neurocognitive disorders in the neonatal rat brain, but the full extent of damage induced by anesthesia on the central nervous system is still unknown.
We aim to investigate whether sevoflurane anesthesia affects the spinal cord.
After the approval of the ethics committee, 24 Wistar albino rat pups, weighing between 9 g and 11 g, on the postnatal 7th day were included in the study. In the sevoflurane groups, rats breathed 2.5% sevoflurane in oxygen. The tail flick tests were performed on postnatal 8th, 15th, and 30th days to evaluate motor functions. At the end of the experiments, rats were sacrificed by decapitation, and their spinal cords were taken for histopathological evaluation.
There was a significant difference between the tail pulling times on the 8th and 30th days in both groups ( = 0.036). No significant difference was found between the control and sevoflurane groups ( = 0.053). In histopathological assessments, the chronic sevoflurane group showed a significant increase in apoptotic cell count ( < 0.001).
This study showed that although there was a significant increase in apoptotic cells in the chronic sevoflurane group, motor function of the spinal cord was not affected. Further studies can be conducted to investigate the possible mechanisms.
麻醉已被证明与新生大鼠大脑中的神经细胞凋亡及长期神经认知障碍有关，但麻醉对中枢神经系统造成损害的全貌仍不清楚。
我们旨在研究七氟醚麻醉是否会影响脊髓。
在伦理委员会批准后，将24只出生后第7天、体重在9克至11克之间的Wistar白化病幼鼠纳入研究。在七氟醚组中，大鼠吸入含2.5%七氟醚的氧气。在出生后第8天、第15天和第30天进行甩尾试验以评估运动功能。实验结束时，通过断头处死大鼠，并取出其脊髓进行组织病理学评估。
两组在第8天和第30天的甩尾时间存在显著差异（P = 0.036）。对照组和七氟醚组之间未发现显著差异（P = 0.053）。在组织病理学评估中，慢性七氟醚组的凋亡细胞计数显著增加（P < 0.001）。
本研究表明，虽然慢性七氟醚组的凋亡细胞显著增加，但脊髓的运动功能未受影响。可开展进一步研究以探究其可能机制。
