Yin Jiaming, Zhao Jiajia, Wang Zeng, Fang Zhen, Guo Huiming, Cheng Hongmei, Li Jie, Shen Jie, Yin Meizhen, Su Xiaofeng, Yan Shuo
Frontiers Science Center for Molecular Design Breeding, Department of Plant Biosecurity, College of Plant Protection, China Agricultural University, Beijing, 100193, China.
Sanya Institute of China Agricultural University, Sanya, 572025, China.
Adv Sci (Weinh). 2024 Dec;11(48):e2410585. doi: 10.1002/advs.202410585. Epub 2024 Nov 18.
Nanomaterials cannot only act as active ingredients (AIs), but also adjuvants to encapsulate or attach AIs to improve their fungicidal activity. Herein, a hydrophilic and lipophilic diblock polymer (HLDP) is designed and synthesized to prepare a series of HLDP nano-protectants to explore the best HLDP nano-protectant for anthracnose management. These results demonstrate that the HLDP-CS nano-protectant displays the best control effects on mango anthracnose via the direct pathogen inhibition and amplified plant immune responses. The HLDP can be spontaneously conjugated with CS into nanoscale spherical particles through hydrophobic interaction. The complexation of CS with HLDP remarkably improves the deposition and adhesion of CS droplets on mango leaves. The HLDP can interact with mycelium via electrostatic interaction to damage the cell wall/membrane, which can act as an AI to directly suppress the spore germination and mycelial growth. Meanwhile, HLDP can be applied as an adjuvant for CS to amplify the plant immune responses via accelerating the biosynthesis of secondary metabolites and plant hormones. This work reports the multiple missions for nanomaterials in pathogen control, which proposes a novel strategy for designing nano-protectant with dual-synergistic mechanism.
纳米材料不仅可以作为活性成分，还可以作为佐剂来包裹或附着活性成分以提高其杀菌活性。在此，设计并合成了一种亲水性和疏水性双嵌段聚合物（HLDP），以制备一系列HLDP纳米保护剂，探索用于炭疽病防治的最佳HLDP纳米保护剂。这些结果表明，HLDP-CS纳米保护剂通过直接抑制病原体和增强植物免疫反应，对芒果炭疽病表现出最佳的防治效果。HLDP可通过疏水相互作用与壳聚糖自发共轭形成纳米级球形颗粒。壳聚糖与HLDP的络合显著提高了壳聚糖液滴在芒果叶片上的沉积和附着力。HLDP可通过静电相互作用与菌丝体相互作用，破坏细胞壁/细胞膜，可作为一种活性成分直接抑制孢子萌发和菌丝体生长。同时，HLDP可作为壳聚糖的佐剂，通过加速次生代谢产物和植物激素的生物合成来增强植物免疫反应。这项工作报道了纳米材料在病原体控制中的多重作用，为设计具有双重协同机制的纳米保护剂提出了一种新策略。
