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[酶联免疫吸附测定法在细菌学和病毒学中的当前作用]

[Current role of ELISA methods in bacteriology and virology].

作者信息

Ramuz M

出版信息

Ann Biol Clin (Paris). 1985;43(2):163-5.

PMID:4073608
Abstract

The use of fixed-phase immuno-enzymatic methods (ELISA) is becoming more widespread. In theory, these methods appear to be attractive because of their adaptability to various clinical situations. However, in practice problems remain to be solved. The detection of serum antibodies would seem to be easier, with the exception of tests for IgM, which are interfered with by rheumatoid factor. The detection of viral or bacterial antigens in pathological material would seem to be more difficult to achieve, but holds great promise for the future. Improvements have already been made, but progress remains to be made in the technique and in the clinical interpretation of the results.

摘要

固定相免疫酶法(酶联免疫吸附测定法,ELISA)的应用正变得越来越广泛。理论上,这些方法因其对各种临床情况的适应性而显得颇具吸引力。然而,在实际应用中,仍有问题有待解决。血清抗体的检测似乎相对容易,不过检测IgM的试验除外,类风湿因子会干扰此类检测。在病理材料中检测病毒或细菌抗原似乎更难实现,但未来前景广阔。虽然已经取得了一些进展,但在技术以及结果的临床解读方面仍有待进一步改进。

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