Chang Che Lun, Chang Shen Chang, Lin Li Jen, Chang Jhih Siang, Lin Min Jung, Lee Tzu Tai
Department of Animal Science, National Chung Hsing University, Taichung 402, Taiwan.
Southern Region Branch, Taiwan Livestock Research Institute, Ministry of Agriculture, Pingtung 91201, Taiwan; Department of Animal Science, National Chiayi University, Chiayi 600, Taiwan.
Poult Sci. 2025 Jul 18;104(10):105584. doi: 10.1016/j.psj.2025.105584.
The purpose of this study was to evaluate black soldier fly pupal exuviae (BSFP) fermented by Bacillus amyloliquefaciens (FBSFP) as a functional feed additive for Red Feather Native chickens. Moreover, it investigated the effects of the feed additive on growth performance, blood parameters, intestinal morphology, and microbiota composition. These chickens were allocated into five groups and their feeds are as follows: basal diet (control), basal diet supplemented with 1 × 10 CFU/kg BA (BA), 0.5 % BSFP (BSFP2), 0.25 % FBSFP (contains 5 × 10 CFU/kg BA, FBSFP1), and 0.5 % FBSFP (contains 1 × 10 CFU/kg BA, FBSFP2). The experiment lasted for 70 days. Although there were no differences in the growth performance (1-70 d) among the five groups, the count of lactic acid bacteria in the ileum increased significantly in all groups, while the coliform counts decreased significantly in BA group compared to the control group. FBSFP1 resulted in higher jejunal villi height and crypt depth ratio. All treatments decreased the serum MDA contents of chickens. However, the serum catalase and glutathione peroxidase activities of FBSFP2 were significantly higher than those of other groups. Supplementation with FBSFP could significantly increase serum tumor necrosis factor-α levels in chickens and significantly decreased the lightness (L*) of breast muscle, meanwhile, significantly reducing the cooking loss of thigh muscle at the 0.5 % supplementation. Except for the BSFP group, supplementation with both BA and FBSFP (0.25 % and 0.5 %) in the diets decreased the counts of coliform bacteria and indole concentration in the litter. In conclusion, FBSFP demonstrated great potential as a feed additive for chickens by improving the intestinal microflora, morphology, and antioxidant capacity of chickens, and offered positive effects on litter quality.
本研究旨在评估解淀粉芽孢杆菌发酵的黑水虻蛹蜕（FBSFP）作为红羽土鸡功能性饲料添加剂的效果。此外，还研究了该饲料添加剂对生长性能、血液参数、肠道形态和微生物群组成的影响。将这些鸡分为五组，其饲料如下：基础日粮（对照组）、添加1×10 CFU/kg BA的基础日粮（BA）、0.5%黑水虻蛹蜕（BSFP2）、0.25% FBSFP（含5×10 CFU/kg BA，FBSFP1）和0.5% FBSFP（含1×10 CFU/kg BA，FBSFP2）。实验持续70天。虽然五组之间的生长性能（1 - 70天）没有差异，但所有组回肠中乳酸菌数量显著增加，而与对照组相比，BA组的大肠菌数量显著减少。FBSFP1导致空肠绒毛高度与隐窝深度比值更高。所有处理均降低了鸡的血清丙二醛含量。然而，FBSFP2组的血清过氧化氢酶和谷胱甘肽过氧化物酶活性显著高于其他组。添加FBSFP可显著提高鸡血清肿瘤坏死因子-α水平，并显著降低胸肌亮度（L*），同时，在添加量为0.5%时显著降低大腿肌肉的蒸煮损失。除BSFP组外，日粮中添加BA和FBSFP（0.25%和0.5%）均降低了垫料中大肠菌数量和吲哚浓度。总之，FBSFP通过改善鸡的肠道微生物群、形态和抗氧化能力，显示出作为鸡饲料添加剂的巨大潜力，并对垫料质量产生积极影响。
