利用高压液相色谱法快速测定血清中卡那霉素和地贝卡星的方法。
Rapid method for determination of kanamycin and dibekacin in serum by use of high-pressure liquid chromatography.
作者信息
Kubo H, Kobayashi Y, Nishikawa T
出版信息
Antimicrob Agents Chemother. 1985 Oct;28(4):521-3. doi: 10.1128/AAC.28.4.521.
Abstract
A rapid, simple, and accurate method for the determination of kanamycin and dibekacin in serum by use of high-pressure liquid chromatography is described. The serum proteins were precipitated with 3.5% perchloric acid containing sodium octanesulfonate. After centrifugation, a sample of the supernatant was directly injected into the chromatograph. The determination of kanamycin and dibekacin was performed by a combination of reverse-phase, ion-pair chromatography, postcolumn derivatization with o-phthalaldehyde, and fluorescence detection. The correlation coefficients with fluorescence polarization immunoassay were 0.996 for kanamycin and 0.957 for dibekacin.
摘要
描述了一种使用高压液相色谱法快速、简单且准确地测定血清中卡那霉素和地贝卡星的方法。血清蛋白用含辛烷磺酸钠的3.5%高氯酸沉淀。离心后，将上清液样品直接注入色谱仪。卡那霉素和地贝卡星的测定采用反相、离子对色谱法、邻苯二甲醛柱后衍生化和荧光检测相结合的方法。与荧光偏振免疫测定法的相关系数，卡那霉素为0.996，地贝卡星为0.957。
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Rapid method for determination of kanamycin and dibekacin in serum by use of high-pressure liquid chromatography.利用高压液相色谱法快速测定血清中卡那霉素和地贝卡星的方法。
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