采用高压液相色谱法测定血清中的庆大霉素。
Assay of gentamicin in serum by high-pressure liquid chromatography.
作者信息
Anhalt J P
出版信息
Antimicrob Agents Chemother. 1977 Apr;11(4):651-5. doi: 10.1128/AAC.11.4.651.
Abstract
A procedure is described that uses high-pressure liquid chromatography for assay of gentamicin in serum. The technique involves extraction of gentamicin from serum by using a CM-Sephadex column and analysis by reverse-phase, ion-pair chromatography. Continuous-flow, post-column derivatization with o-phthaladehyde is used to form fluorescent products for detection. The possible scope of this method for analysis of other aminoglycoside antibiotics is illustrated by application to sisomicin and netilmicin.
摘要
本文描述了一种使用高压液相色谱法测定血清中庆大霉素的方法。该技术包括通过使用CM - 葡聚糖凝胶柱从血清中提取庆大霉素，并通过反相离子对色谱法进行分析。采用邻苯二醛进行连续流动柱后衍生化以形成荧光产物用于检测。通过应用于西索米星和奈替米星说明了该方法分析其他氨基糖苷类抗生素的可能范围。
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Assay of gentamicin in serum by high-pressure liquid chromatography.采用高压液相色谱法测定血清中的庆大霉素。
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Fluorescence reaction for amino acids.氨基酸的荧光反应。
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Chemical determination of component ratio and potency of gentamicin complex.庆大霉素复合物成分比例及效价的化学测定
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O-phthalaldehyde: fluorogenic detection of primary amines in the picomole range. Comparison with fluorescamine and ninhydrin.邻苯二甲醛：皮摩尔范围内伯胺的荧光检测。与荧光胺和茚三酮的比较。
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