采用高压液相色谱法测定血清中的庆大霉素。
Assay of gentamicin in serum by high-pressure liquid chromatography.
作者信息
Anhalt J P
出版信息
Antimicrob Agents Chemother. 1977 Apr;11(4):651-5. doi: 10.1128/AAC.11.4.651.
Abstract
A procedure is described that uses high-pressure liquid chromatography for assay of gentamicin in serum. The technique involves extraction of gentamicin from serum by using a CM-Sephadex column and analysis by reverse-phase, ion-pair chromatography. Continuous-flow, post-column derivatization with o-phthaladehyde is used to form fluorescent products for detection. The possible scope of this method for analysis of other aminoglycoside antibiotics is illustrated by application to sisomicin and netilmicin.
摘要
本文描述了一种使用高压液相色谱法测定血清中庆大霉素的方法。该技术包括通过使用CM - 葡聚糖凝胶柱从血清中提取庆大霉素，并通过反相离子对色谱法进行分析。采用邻苯二醛进行连续流动柱后衍生化以形成荧光产物用于检测。通过应用于西索米星和奈替米星说明了该方法分析其他氨基糖苷类抗生素的可能范围。
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Assay of gentamicin in serum by high-pressure liquid chromatography.采用高压液相色谱法测定血清中的庆大霉素。
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本文引用的文献
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Fluorescence reaction for amino acids.氨基酸的荧光反应。
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Chemical determination of component ratio and potency of gentamicin complex.庆大霉素复合物成分比例及效价的化学测定
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Separation of gentamicin complex by ion-exchange column chromatography.通过离子交换柱色谱法分离庆大霉素复合物。
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Ion-pair extraction and high-speed liquid chromatography of cephalothin and deacetylcephalothin in human serum and urine.人血清和尿液中头孢噻吩及去乙酰头孢噻吩的离子对萃取与高效液相色谱法
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High-performance liquid chromatographic determination of kanamycin.
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Reversed-phase ion-pair partition chromatography of carboxylates and sulphonates.羧酸盐和磺酸盐的反相离子对分配色谱法。
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Assay of flucytosine and furosemide by high-pressure liquid chromatography.高效液相色谱法测定氟胞嘧啶和呋塞米
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