Du Yang, Li Guangshuo, Ji Zeqiang, Wang Dandan, Wu Jianwei, Liu Yanfang, Kang Kaijiang, Zhao Xingquan, Bian Liheng
Department of Neurology, Fengtai District, Beijing Tiantan Hospital, Capital Medical University, No. 119 Nansihuanxilu, Beijing, 100070, China.
China National Clinical Research Center for Neurological Diseases, Beijing, China.
J Neuroinflammation. 2025 Jul 30;22(1):195. doi: 10.1186/s12974-025-03518-z.
As limited medical interventions proved efficacy in intracerebral hemorrhage (ICH), the mechanisms underlying primary and secondary tissue injury remain poorly understood based on hematoma. In this study, we aimed to investigate the transcriptional profiles of immune cells from hematoma samples derived from ICH patients, which was rarely focused before.
Single cell RNA sequencing (scRNA-seq) was performed on hematoma and blood samples derived from ICH patients, with blood samples from healthy volunteers serving as a control. Subsequent comprehensive analyses, including cell-cell communication and trajectory inference, were conducted to provide a detailed understanding of immune cell characteristics in ICH.
Hematoma and blood samples were derived from four ICH patients, with blood samples from three healthy volunteers as control. At the single-cell level, three major immune cell types were identified, with neutrophils playing a predominant role. Subtypes of these immune cells were further characterized based on their profiles in hematoma and blood samples. The role of Toll-like receptor 4 in hematoma in ICH was further investigated from the perspective of immune inflammation and metabolism.
This study offers a comprehensive overview of the immune cell landscape in ICH, derived from hematoma and blood samples, which has been rarely reported. The dynamic inflammatory processes involving distinct immune cell types were further elucidated, and provides potential therapeutic targets for future interventions.
由于有限的医学干预已证明对脑出血（ICH）有效，但基于血肿的原发性和继发性组织损伤的潜在机制仍知之甚少。在本研究中，我们旨在研究来自ICH患者血肿样本的免疫细胞转录谱，这在以前很少受到关注。
对来自ICH患者的血肿和血液样本进行单细胞RNA测序（scRNA-seq），以健康志愿者的血液样本作为对照。随后进行了包括细胞间通讯和轨迹推断在内的综合分析，以详细了解ICH中免疫细胞的特征。
血肿和血液样本来自4例ICH患者，以3例健康志愿者的血液样本作为对照。在单细胞水平上，鉴定出三种主要免疫细胞类型，其中中性粒细胞起主要作用。根据这些免疫细胞在血肿和血液样本中的特征进一步对其亚型进行了表征。从免疫炎症和代谢的角度进一步研究了Toll样受体4在ICH血肿中的作用。
本研究全面概述了来自血肿和血液样本的ICH免疫细胞格局，这在以往很少有报道。进一步阐明了涉及不同免疫细胞类型的动态炎症过程，并为未来的干预提供了潜在的治疗靶点。
