Striae distensae, commonly known as stretch marks, are dermal scars resulting from excessive skin stretching and structural remodeling. Despite numerous treatment modalities, patient satisfaction varies significantly, and effective management remains a challenge. This study aimed to evaluate patient satisfaction with novel serum containing amino acids, vitamins, and growth factors for the treatment of striae distensae and to assess the impact of individual characteristics, lesion aspects, and patient habits on treatment outcomes. A total of 156 patients underwent MN-assisted application of the serum. Treatment frequency and duration were determined by physicians on the basis of individual response. Patients completed an online questionnaire assessing demographics, lesion characteristics, lifestyle habits, and satisfaction via the customer satisfaction score (CSAT). Data were analyzed to identify factors influencing treatment satisfaction. Overall, 82.4% of patients were very satisfied, and 78.4% reported significant improvement in striae appearance. Satisfaction was highest in younger patients (≤ 44 years), those with newer striae, and nonsmokers. Patients with medium skin tones reported greater satisfaction than did those with very fair or dark brown/black skin tones. Most patients (65%) required only one treatment session to observe improvements, and 72% noticed visible results within two weeks. Adverse effects were minimal, affecting only 8.4% of the participants. The serum demonstrated high patient satisfaction and rapid improvement in the striae distensae. Factors such as age, striae duration, smoking, and skin tone influenced treatment outcomes. These findings support the importance of personalized treatment approaches for optimal results.