Liu Jiahui, Wu Yuanyuan, Lin Jinru, Xie Mengxia, Chen Likai, Wang Liguo
Laboratory of Germplasm Resources and Molecular Identification of Traditional Chinese Medicine, School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China.
Artemisinin Research Center, Guangzhou University of Chinese Medicine, Guangzhou, China.
Front Microbiol. 2025 Jul 16;16:1536079. doi: 10.3389/fmicb.2025.1536079. eCollection 2025.
Willd is a perennial herbaceous plant that usually has a stable microflora living in the inter-root and stem and leaf tissues, which assists the host in normal growth and development. The bacterial wilt disease investigated in planting bases is a novel soil-borne disease caused by the pathogenic bacterium (Smith) Yabuuchi et al., which disrupts the -microbe-soil microecological balance. For this reason, it is important to study the changes in endophytic fungal community growth and diversity in healthy and diseased , and to mine the active endophytic fungal resources in order to lay the foundation for exploring the functional microbial communities for artificial synthesis. From 685 endophytic fungi strains isolated from healthy (HDK_J) and diseased (HDK_B) stems/leaves, 27 species were identified in HDK_J and 8 in HDK_B (belonging to 3 Phyla, 6 Classes, 13 Families). HDK_B's fungal relative abundance (RA) was only 38.93% of HDK J's, indicating significantly lower composition/abundance. While species in stems and leaves were identical within health groups, leaf RA exceeded stems by 124.23% in HDK_J and 78.23% in HDK_B. The RA of HDK_J leaves was 78.08% higher than that of stems. All diversity indices for HDK_J were higher than those for HDK_B, with significant differences. The phylogenetic trees revealed four major branches of endophytic fungi species in HDK_J, and especially, there were many long development branches under the Ascomycota. In contrast, the phylogenetic tree for HDK_B showed only one major branch (Ascomycota) with few sub-branches. The bacterial wilt significantly affected the composition and RA of endophytic fungi in . The diversity indices showed a decreasing trend in after being infected by . The dominant species were changed. The parts of sensitive endophytic fungi had disappeared. This result will be helpful for studies on the relationship between the artificial minimal microbial community and the role of the host, as well as for studies on synthetic microbiomics.
野生是一种多年生草本植物，其根际以及茎和叶组织中通常生活着稳定的微生物群落，有助于宿主正常生长发育。在种植基地调查的青枯病是由病原菌（史密斯）矢布uchi等人引起的一种新型土传病害，它破坏了微生物-土壤微生态平衡。因此，研究健康和患病野生植物内生真菌群落生长和多样性的变化，挖掘活性内生真菌资源，为探索人工合成功能微生物群落奠定基础具有重要意义。从健康（HDK_J）和患病（HDK_B）野生植物茎/叶中分离出的685株内生真菌菌株中，HDK_J鉴定出27种，HDK_B鉴定出8种（属于3个门、6个纲、13个科）。HDK_B的真菌相对丰度（RA）仅为HDK_J的38.93%，表明其组成/丰度显著较低。虽然健康组内茎和叶中的物种相同，但HDK_J中叶的RA比茎高124.23%，HDK_B中叶的RA比茎高78.23%。HDK_J叶的RA比茎高78.08%。HDK_J的所有多样性指数均高于HDK_B，差异显著。系统发育树揭示了HDK_J中内生真菌物种的四个主要分支，特别是在子囊菌门下有许多长的发育分支。相比之下，HDK_B的系统发育树仅显示一个主要分支（子囊菌门），分支较少。青枯病显著影响野生植物内生真菌的组成和RA。多样性指数显示，野生植物感染青枯病后呈下降趋势。优势物种发生了变化。敏感内生真菌部分消失。这一结果将有助于研究人工最小微生物群落与宿主作用之间的关系，以及合成微生物组学研究。
