Vegetable Institute of Beijing Academy of Agriculture and Forestry Sciences, Beijing, China.
North China Key Laboratory of Urban Agriculture, Ministry of Agriculture, Beijing, China.
PLoS One. 2024 Aug 16;19(8):e0298910. doi: 10.1371/journal.pone.0298910. eCollection 2024.
The closed nutrient solution management method allows for the recycling and utilization of nutrient solutions, improving the efficiency of water and fertilizer utilization. This study was conducted to investigate the effects of changing the frequency of nutrient solution renewal and method of nutrient supply on the microbial communities composition, yield, and quality in closed soilless systems by using high-throughput sequencing technology and combining the physicochemical properties of root exudate solution. The results showed that different nutrient solution management modes had a significant impact on the structure and diversity of root exudate solution microbial communities. The abundance and diversity of microorganisms in inorganic perlites were correlative with EC. The abundance and diversity of bacterial communities in the root exudate solution of open liquid supply (CK) were higher than that of closed liquid supply, while the abundance and diversity of fungal communities in the root exudate solution of closed liquid supply (T1, T2, T3) were higher than that of open liquid supply. As the frequency of nutrient solution interval decreased, the accumulation of salt in root exudate solution and the richness and diversity of the fungal community also decreased, especially increasing the K+, Ca2+, and Mg2+ contents, which were positively correlated with potential beneficial Candidatus_Xiphinematobacter, Arachidicoccus, Cellvibrio, Mucilaginibacter, Taibaiella communities and decreasing the content of soluble protein, Vitamin C content, but not significantly increased cucumber yield.
封闭式营养液管理方法可实现营养液的循环利用，提高水肥利用效率。本研究采用高通量测序技术，结合根分泌物的理化性质，研究了改变营养液更新频率和供液方式对封闭式无土系统中微生物群落组成、产量和品质的影响。结果表明，不同营养液管理方式对根分泌物微生物群落的结构和多样性有显著影响。无机珍珠岩中微生物的丰度和多样性与 EC 呈正相关。开放式液供应（CK）根分泌物中细菌群落的丰度和多样性高于封闭式液供应，而封闭式液供应（T1、T2、T3）根分泌物中真菌群落的丰度和多样性高于开放式液供应。随着营养液间隔时间的减少，根分泌物中盐分的积累以及真菌群落的丰富度和多样性也随之减少，特别是增加 K+、Ca2+和 Mg2+的含量，这与潜在有益的 Candidatus_Xiphinematobacter、Arachidicoccus、Cellvibrio、Mucilaginibacter、Taibaiella 群落呈正相关，同时降低可溶性蛋白和维生素 C 含量，但对黄瓜产量没有显著提高。
