Takeda Yohei, Jamsransuren Dulamjav, Makita Yoshimasa, Matsuda Sachiko, Ogawa Haruko, Oh Hourei
Research Center for Global Agromedicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
Department of Veterinary Medicine, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Japan.
J Water Health. 2025 Jul;23(7):894-904. doi: 10.2166/wh.2025.009. Epub 2025 Jul 7.
Human adenoviruses (AdVs) are non-enveloped viruses that cause respiratory, ocular, and gastrointestinal infections. Owing to the absence of commercialized antiviral drugs for AdV infection, the urgent prevention of infection by inactivating AdVs is pertinent to public health. Accordingly, to establish effective disinfection methods against AdVs, this study assessed and compared the virucidal efficacy of multiple virucidal agents, namely ozonated water (OW), povidone-iodine (PVPI), and ethanol (EtOH), against AdV type 40 . The AdV-inactivating activity of OW was influenced by ozone concentration and was adversely affected by the presence of proteins. PVPI demonstrated consistent virucidal activity across a concentration range of 0.025-0.050% and maintained its efficacy in the presence of proteins. EtOH showed time-dependent and limited concentration-dependent virucidal activity across a concentration range of 60-80% and maintained its effectiveness in the presence of proteins. Polymerase chain reaction analysis of the viral genome revealed that OW was the only agent among the three that induced AdV genome degradation. Therefore, the study findings indicate that if the properties of OW are comprehensively understood to ensure its appropriate use, OW can be applied as a useful virucidal agent against AdV as well as other virus-inactivating agents, such as PVPI and EtOH.
人腺病毒（AdV）是一种无包膜病毒，可引起呼吸道、眼部和胃肠道感染。由于缺乏针对腺病毒感染的商业化抗病毒药物，通过灭活腺病毒来紧急预防感染对于公共卫生至关重要。因此，为了建立有效的针对腺病毒的消毒方法，本研究评估并比较了多种杀病毒剂，即臭氧水（OW）、聚维酮碘（PVPI）和乙醇（EtOH）对40型腺病毒的杀病毒效果。臭氧水的腺病毒灭活活性受臭氧浓度影响，并且会因蛋白质的存在而受到不利影响。聚维酮碘在0.025 - 0.050%的浓度范围内表现出一致的杀病毒活性，并且在有蛋白质存在的情况下仍保持其效力。乙醇在60 - 80%的浓度范围内表现出时间依赖性和有限的浓度依赖性杀病毒活性，并且在有蛋白质存在的情况下仍保持其有效性。对病毒基因组的聚合酶链反应分析表明，臭氧水是这三种试剂中唯一能诱导腺病毒基因组降解的试剂。因此，研究结果表明，如果全面了解臭氧水的特性以确保其正确使用，臭氧水可作为一种有效的针对腺病毒的杀病毒剂以及其他病毒灭活剂，如聚维酮碘和乙醇。
