Dr Brill and Partner GmbH, Institute for Hygiene and Microbiology, Bremen, Germany.
Laboratorium für Virusforschung, University Bremen/UFT, Bremen, Germany.
Am J Infect Control. 2023 Sep;51(9):1011-1016. doi: 10.1016/j.ajic.2023.01.014. Epub 2023 Feb 2.
Adenoviruses belong to the stable nonenveloped viruses playing an important role in healthcare-associated infections mainly causing respiratory infections and epidemic keratoconjunctivitis. Hand disinfection with alcoholic preparations is therefore one of the most important measures to prevent such viral infections in hospitals and other medical settings.
The inactivation of adenovirus type 5 by ethanol, 1- and 2-propanol, and 2 commercially available hand disinfectants was examined at different concentrations, temperatures, and pH-values.
For ethanol and 1-propanol the maximum virus-inactivating properties after 30 seconds exposure were found at a concentration of 60%-70% and 50%-60%, respectively, whereas with 2-propanol no activity was observed. The virucidal activity of all alcohols and the 2 hand disinfectants examined was increased when raising the temperature from 20°C to 25°C. By increasing the pH value to 9, a strong improvement of the activity of ethanol, 1-propanol and 1 hand disinfectant was observed, whereas pH lowering resulted in decrease of activity.
These results demonstrate the importance of physical parameters in the inactivation of adenoviruses by alcohols and will help to improve measures to reduce adenovirus transmission in healthcare settings.
腺病毒属于稳定的非包膜病毒，在医疗机构相关感染中起着重要作用，主要引起呼吸道感染和流行性角膜结膜炎。因此，手部用酒精制剂消毒是预防医院和其他医疗环境中此类病毒感染的最重要措施之一。
在不同浓度、温度和 pH 值下，检测了乙醇、1-丙醇和 2-丙醇以及两种市售手部消毒剂对 5 型腺病毒的灭活作用。
对于乙醇和 1-丙醇，在暴露 30 秒后，最大的病毒灭活特性分别出现在浓度为 60%-70%和 50%-60%，而 2-丙醇则没有活性。所有醇类和两种手部消毒剂的杀病毒活性在温度从 20°C 升高到 25°C 时均有所提高。将 pH 值提高到 9 可显著增强乙醇、1-丙醇和一种手部消毒剂的活性，而降低 pH 值则会降低其活性。
这些结果表明了物理参数在酒精灭活腺病毒中的重要性，并将有助于改进减少医疗机构中腺病毒传播的措施。
