Chamberlain Connie S, Tremmel Daniel M, Khan Ayesha Palwasha, Santos Ellen Abad, Kapadia Deep, Leavens Caterra, Gorski Katherine M, Hamadeh Rawan, Feeney Austin K, Odorico Jon, Sackett Sara D
Division of Transplantation, Department of Surgery, University of Wisconsin-Madison, Madison, WI 53705, USA.
Division of Transplantation, Department of Surgery, University of Wisconsin-Madison, Madison, WI 53705, USA.
STAR Protoc. 2025 Jul 29;6(3):103997. doi: 10.1016/j.xpro.2025.103997.
We present a protocol for transforming human pancreatic tissue into a decellularized and delipidized extracellular matrix (ECM) for hydrogel/scaffold applications. We describe steps for decellularization and delipidization using sodium deoxycholate and sequential ethanol, acetone, and hexane washes and removal of nucleic acids with benzonase. We then detail procedures for lyophilizing the ECM for long-term preservation and digesting the pancreatic ECM in pepsin-HCL to form a hydrogel. For complete details on the use and execution of this protocol, please refer to Tremmel et al. and Sackett et al..
我们提出了一种将人胰腺组织转化为用于水凝胶/支架应用的脱细胞和脱脂细胞外基质（ECM）的方案。我们描述了使用脱氧胆酸钠以及依次用乙醇、丙酮和己烷洗涤进行脱细胞和脱脂的步骤，以及用核酸酶去除核酸的步骤。然后，我们详细说明了将ECM冻干以进行长期保存以及在胃蛋白酶 - 盐酸中消化胰腺ECM以形成水凝胶的程序。有关此方案的使用和执行的完整详细信息，请参考Tremmel等人和Sackett等人的研究。
