Paulsson M, Dziadek M, Suchanek C, Huttner W B, Timpl R
Biochem J. 1985 Nov 1;231(3):571-9. doi: 10.1042/bj2310571.
Seven different sulphated macromolecules were detected in 6 M-guanidinium chloride extracts of metabolically [35S]sulphate-labelled mouse Reichert's membrane and were partially separated. Polypeptide bands of apparent Mr 50 000, 150 000 (tentatively identified as entactin) and 170 000 contained essentially tyrosine O-sulphate as the labelled component. Most of the radioactive sulphate was incorporated into three different proteoglycans, which could be separated by chromatography and density-gradient centrifugation before and after enzymic degradation. Enzymic analysis of glycosaminoglycans and of protein cores by immunoassays identified these components as low-density and high-density forms of heparan sulphate proteoglycan and a high-density form of chondroitin sulphate or dermatan sulphate proteoglycan.
在经代谢性[35S]硫酸盐标记的小鼠雷氏膜的6M氯化胍提取物中检测到7种不同的硫酸化大分子，并对其进行了部分分离。表观分子量为50000、150000（暂定为巢蛋白）和170000的多肽带主要含有酪氨酸O-硫酸盐作为标记成分。大部分放射性硫酸盐被整合到三种不同的蛋白聚糖中，这些蛋白聚糖在酶解前后可通过色谱法和密度梯度离心法分离。通过免疫测定对糖胺聚糖和蛋白核心进行酶分析，确定这些成分分别为低密度和高密度形式的硫酸乙酰肝素蛋白聚糖以及高密度形式的硫酸软骨素或硫酸皮肤素蛋白聚糖。
