Semoff S, Hogan B L, Hopkins C R
EMBO J. 1982;1(10):1171-5. doi: 10.1002/j.1460-2075.1982.tb00009.x.
Immunoelectron microscopy using protein A-colloidal gold complexes of different sizes was used to study the relative distribution of extracellular matrix glycoproteins within Reichert's membrane (RM) of 13.5-day mouse embryos. Labelling for fibronectin was distributed asymmetrically; the highest concentration occurring in the outermost layer adjacent to the trophoblast cells and negligible labelling in the inner matrix, beneath the parietal endoderm cells. Within the main body of the membrane, fibronectin was concentrated in discrete electron-opaque deposits. Antibodies raised against the native complex between laminin and entactin , and against entactin alone labelled the RM more uniformly.
使用不同大小的蛋白A-胶体金复合物进行免疫电子显微镜研究，以探讨13.5天龄小鼠胚胎的瑞氏膜（RM）中细胞外基质糖蛋白的相对分布。纤连蛋白的标记分布不对称；最高浓度出现在与滋养层细胞相邻的最外层，而在内胚层细胞下方的内部基质中标记可忽略不计。在膜的主体内，纤连蛋白集中在离散的电子不透明沉积物中。针对层粘连蛋白和巢蛋白之间的天然复合物以及单独针对巢蛋白产生的抗体对RM的标记更为均匀。
