Yang Zehao, Chen Yanyu, Fang Zhen, Jia Kehan, Shi Wangpeng, Shen Jie, Wang Dan
State Key Laboratory of Agricultural and Forestry Biosecurity, MARA Key Lab of Surveillance and Management for Plant Quarantine Pests, College of Plant Protection, China Agricultural University, Beijing 100193, China; Sanya Institute of China Agricultural University, Sanya 572025, China.
Sanya Institute of China Agricultural University, Sanya 572025, China; Institute of Tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences, Haikou 571101, China.
Pestic Biochem Physiol. 2025 Sep;213:106507. doi: 10.1016/j.pestbp.2025.106507. Epub 2025 Jun 11.
Entomopathogenic fungi are eco-friendly biological method to control various agricultural pests. However, it takes time during the practical application of Beauveria bassiana in thrip management. This study investigates the role of Megalurothrips usitatus Dorsal switch protein 1 (MuDSP1), which is an immune regulatory gene during fungus infection. MuDSP1 is highly expressed at the pupal and adult stages in M. usitatus. Following the infection with B. bassiana, the expression of MuDSP1 is upregulated, as well as Phospholipase A2, lysozyme, and apolipophorin in the immune pathway. The later three genes were repressed by the interference of MuDSP1, confirming the crucial role of MuDSP1 in the immune response. Interference of MuDSP1 has no effects on the mortality of bean flower thrips, but the combination of MuDSP1 dsRNA and B. bassiana infection significantly increases the mortality caused by B. bassiana. This could be due to the attenuated immune response induced by MuDSP1 dsRNA. In conclusion, this study reveals the significant role of MuDSP1 in immune response to fungi. Simultaneous application of MuDSP1 dsRNA and B. bassiana not only provides a new strategy for high-efficient biological management but also offers an alternative approach to sustainable agricultural pest control.
昆虫病原真菌是控制各种农业害虫的生态友好型生物方法。然而，球孢白僵菌在蓟马防治的实际应用中需要时间。本研究调查了豆大蓟马背侧开关蛋白1（MuDSP1）的作用，它是真菌感染期间的一种免疫调节基因。MuDSP1在豆大蓟马的蛹期和成虫期高度表达。用球孢白僵菌感染后，MuDSP1以及免疫途径中的磷脂酶A2、溶菌酶和载脂蛋白的表达上调。后三个基因受到MuDSP1干扰的抑制，证实了MuDSP1在免疫反应中的关键作用。干扰MuDSP1对豆花蓟马的死亡率没有影响，但MuDSP1双链RNA与球孢白僵菌感染的组合显著增加了球孢白僵菌引起的死亡率。这可能是由于MuDSP1双链RNA诱导的免疫反应减弱。总之，本研究揭示了MuDSP1在对真菌的免疫反应中的重要作用。同时应用MuDSP1双链RNA和球孢白僵菌不仅为高效生物防治提供了新策略，也为可持续农业害虫防治提供了替代方法。
