Ratanavongsiri J, Sriwanthana B, Ubol S, Phanuphak P
Asian Pac J Allergy Immunol. 1985 Dec;3(2):187-90.
Specific cell-mediated immune response (CMIR) against rabies antigens was studied in recipients of two regimens of human diploid cell rabies vaccine (HDCV) using the antigen-stimulated lymphocyte transformation test (LTT) as a measure of CMIR. Reconstituted HDCV could be conveniently used as the in vitro stimulating antigen and the response was antigen-dependent. Conventional intramuscular immunization with full-dose HDCV resulted in positive LTT as early as 14 days after starting immunisation, and peaked on day 28. Intracutaneous immunisation with 0.1 ml of HDCV at four sites on days 0, 3 and 7 was a more efficient means of inducing specific lymphocyte response. Specific CMIR was evident as early as seven days and became maximal on day 14. In addition to the more rapid induction of specific CMIR, our intracutaneous regimen also resulted in a brisker and higher antibody response than the intramuscular regimen. The peak antibody level of the intracutaneous regimen was reached on day 14 whereas that of the intramuscular regimen was reached on day 28 and the geometric mean antibody titre on day 14 of the intracutaneous route was significantly higher than that of the intramuscular regimen. We therefore conclude that our closely spaced intracutaneous immunisation with HDCV was effective both in the induction of specific antibodies and the cell-mediated immune response.
使用抗原刺激淋巴细胞转化试验（LTT）作为细胞介导免疫反应（CMIR）的衡量指标，在接受两种人二倍体细胞狂犬病疫苗（HDCV）接种方案的受试者中研究了针对狂犬病抗原的特异性细胞介导免疫反应。重组HDCV可方便地用作体外刺激抗原，且反应是抗原依赖性的。常规全剂量HDCV肌肉注射免疫早在开始免疫后14天就导致LTT呈阳性，并在第28天达到峰值。在第0、3和7天于四个部位皮内注射0.1 ml HDCV是诱导特异性淋巴细胞反应的更有效方法。特异性CMIR早在第7天就很明显，并在第14天达到最大值。除了更快速地诱导特异性CMIR外，我们的皮内接种方案还比肌肉注射方案产生更强烈、更高的抗体反应。皮内接种方案的抗体峰值水平在第14天达到，而肌肉注射方案在第28天达到，并且皮内途径在第14天的几何平均抗体滴度显著高于肌肉注射方案。因此，我们得出结论，我们采用的紧密间隔皮内接种HDCV在诱导特异性抗体和细胞介导免疫反应方面均有效。
