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固定化猪胃粘蛋白和卵类粘蛋白的亲和层析。一种分离凝集素的通用方法。

Affinity chromatography on immobilized hog gastric mucin and ovomucoid. A general method for isolation of lectins.

作者信息

Freier T, Fleischmann G, Rüdiger H

出版信息

Biol Chem Hoppe Seyler. 1985 Nov;366(11):1023-8. doi: 10.1515/bchm3.1985.366.2.1023.

DOI:10.1515/bchm3.1985.366.2.1023
PMID:4074495
Abstract

Two affinity adsorbents of general applicability for isolating of plant lectins are presented. The isolation of 30 lectins from 27 plants is described. The method works quickly and is inexpensive. In particular, mixtures of different lectins occurring in some plants may be resolved in one run.

摘要

本文介绍了两种适用于分离植物凝集素的通用亲和吸附剂。描述了从27种植物中分离出30种凝集素的过程。该方法操作快速且成本低廉。特别是,一些植物中存在的不同凝集素混合物可以一次性分离出来。

相似文献

1
Affinity chromatography on immobilized hog gastric mucin and ovomucoid. A general method for isolation of lectins.固定化猪胃粘蛋白和卵类粘蛋白的亲和层析。一种分离凝集素的通用方法。
Biol Chem Hoppe Seyler. 1985 Nov;366(11):1023-8. doi: 10.1515/bchm3.1985.366.2.1023.
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Isolation and characterization of a variant of ovomucoid.类卵黏蛋白变体的分离与特性鉴定
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A versatile immunoadsorbent capable of binding lectins of various specificities and its use for the separation of cell populations.一种能够结合多种特异性凝集素的多功能免疫吸附剂及其用于细胞群体分离的用途。
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Application of an ovomucoid-conjugated column for the optical resolution of some pharmaceutically important compounds.卵类粘蛋白偶联柱在某些药学重要化合物的光学拆分中的应用。
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引用本文的文献

1
Plant lectins: occurrence, biochemistry, functions and applications.植物凝集素:存在、生物化学、功能及应用
Glycoconj J. 2001 Aug;18(8):589-613. doi: 10.1023/a:1020687518999.
2
The role of galactosyl-binding lectin in the cellular immune response of the cockroach Periplaneta americana (Dictyoptera).半乳糖结合凝集素在美洲大蠊(蜚蠊目)细胞免疫反应中的作用
Immunology. 1988 Jun;64(2):353-7.
3
Purification of beta-glucuronidase and structural assessment of the carbohydrate chains by lectin affinity immunoelectrophoresis.β-葡萄糖醛酸酶的纯化及通过凝集素亲和免疫电泳对糖链的结构评估。
Glycoconj J. 1991 Aug;8(4):340-9. doi: 10.1007/BF00731346.