Park Hyeonseon, Bae Eunji, Jung Jae Gyeong, Choi Bae Young, Lee Tae-Ho, Shim Donghwan
Department of Biological Science, Chungnam National University, Daejeon, 34134, Republic of Korea.
Forest Biomaterials Research Center, National Institute of Forest Science, Jinju, 52817, Republic of Korea.
Sci Data. 2025 Jul 31;12(1):1335. doi: 10.1038/s41597-025-05702-5.
Zoysia macrostachya Franch. & Sav. is a halophytic perennial turfgrass in the Poaceae family, commonly found in the coastal regions of Korea, Japan, and East Asia. Z. macrostachya thrives in high-salinity environments, making it an excellent model for studying abiotic stress resilience. In this study, we present a chromosome-level genome assembly of Z. macrostachya, constructed using Oxford Nanopore long reads, Illumina short reads, and Omni-C sequencing data. The assembly spans 329.78 Mb across 20 chromosomes, with a scaffold N50 of 19.24 Mb, and includes complete telomeric sequences at both ends. The assembly showed 97.8% complete BUSCOs, indicating high genome completeness. Repeat element and gene annotation identified 44.03% of the genome as repetitive elements and 33,474 protein-coding genes. The gene annotation showed 97.1% complete BUSCOs and 86.92% functionally characterized genes. Macrosynteny analysis highlighted highly collinear relationships with related species, providing a foundational understanding of the Z. macrostachya genomic structure. This high-quality genome serves as a valuable resource for advancing salinity tolerance research and improving the genetic diversity of Zoysia species.
大穗结缕草（Zoysia macrostachya Franch. & Sav.）是禾本科的一种多年生盐生草坪草，常见于韩国、日本和东亚的沿海地区。大穗结缕草在高盐环境中生长旺盛，使其成为研究非生物胁迫抗性的优秀模型。在本研究中，我们展示了大穗结缕草的染色体水平基因组组装，该组装使用牛津纳米孔长读长、Illumina短读长和Omni-C测序数据构建而成。该组装跨越20条染色体，长度为329.78 Mb，支架N50为19.24 Mb，两端均包含完整的端粒序列。该组装显示97.8%的BUSCOs完整，表明基因组完整性高。重复元件和基因注释确定基因组的44.03%为重复元件，有33474个蛋白质编码基因。基因注释显示97.1%的BUSCOs完整，86.92%的基因具有功能特征。宏观共线性分析突出了与相关物种的高度共线性关系，为了解大穗结缕草的基因组结构提供了基础。这个高质量的基因组是推进耐盐性研究和改善结缕草属物种遗传多样性的宝贵资源。
