从堆肥中分离出的木质纤维素降解细菌BK32的全基因组序列数据

Complete genome sequence data of BK32, a lignocellulose-degrading bacterium isolated from compost.

作者信息

Born Pichpunleu, Uke Ayaka, Kosugi Akihiko

机构信息

Biological Resources and Post-Harvest Division, Japan International Research Center for Agricultural Sciences (JIRCAS), 1-1 Ohwashi, Tsukuba, Ibaraki 305-8686, Japan.

Graduate School of Science and Technology, University of Tsukuba, 1-1-1 Tennodai, Tsukuba, Ibaraki 305-8572, Japan.

出版信息

Data Brief. 2025 Jul 11;61:111874. doi: 10.1016/j.dib.2025.111874. eCollection 2025 Aug.

DOI:10.1016/j.dib.2025.111874

PMID:40747440

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12311949/

Abstract

This data article presents the complete genome sequence of BK32, an anaerobic, thermophilic bacterium isolated from compost in Japan. Strain BK32 exhibits enhanced degradation of rice straw compared to the type strain DSM15098. Genome sequencing was performed using PacBio Single Molecule Real-Time (SMRT) technology. De novo assembly revealed a single circular chromosome of 2978,451 bp with a GC content of 29.1%. The genome comprises 2769 protein-coding genes and 113 RNA genes. Annotation highlighted 320 genes involved in carbohydrate metabolism. CAZyme analysis identified genes encoding cellulolytic, hemicellulolytic, and amylolytic enzymes. Strain BK32 degraded over 80% of rice straw, underscoring its potential in lignocellulose saccharification. The complete genome sequence is available in the National Center for Biotechnology Information (NCBI) under accession number CP182825.

摘要

本文献展示了BK32的完整基因组序列，BK32是从日本堆肥中分离出的一种厌氧嗜热细菌。与模式菌株DSM15098相比，BK32菌株对稻草的降解能力更强。使用PacBio单分子实时（SMRT）技术进行基因组测序。从头组装揭示了一条2978451 bp的单环染色体，GC含量为29.1%。该基因组包含2769个蛋白质编码基因和113个RNA基因。注释突出了320个参与碳水化合物代谢的基因。碳水化合物活性酶（CAZyme）分析确定了编码纤维素分解酶、半纤维素分解酶和淀粉分解酶的基因。BK32菌株降解了超过80%的稻草，突出了其在木质纤维素糖化方面的潜力。完整的基因组序列可在国家生物技术信息中心（NCBI）获取，登录号为CP182825。