Shariati Najafabadi Samira, Karizmeh Mohsen Shie, Rafienia Mohammad, Kazemi Mohammad, Dastjerdi Hajar Akbari, Bahramian Hamid, Poursamar Seyed Ali, Reisi Parham, Safaee Azadeh, Amirpour Noushin, Salehi Hossein
Department of Anatomical Sciences and Reproductive Biology, School of Medicine, Isfahan University of Medical Sciences, Hezarjerib street, Isfahan, Iran.
Biomaterials, Nanotechnology, and Tissue Engineering Group, Advanced Medical Technology Department, Isfahan University of Medical Sciences, Isfahan, Iran.
Biomater Sci. 2025 Aug 1. doi: 10.1039/d5bm00588d.
Nerve guidance conduits (NGCs) represent a promising strategy to support axonal growth and orientation during peripheral nerve regeneration. Polycaprolactone (PCL) offers suitable mechanical and biodegradable properties for NGC fabrication. To enhance its functionality, carbon quantum dots (CQDs) can improve physical and chemical properties, while extract contributes bioactive compounds that support neurogenesis and nerve repair. In this study, electrospun aligned nanofiber scaffolds composed of PCL, CQDs, and Moringa extract were fabricated and evaluated. Scaffolds containing both CQDs and Moringa showed improved mechanical strength, hydrophilicity, and degradation profile. The release of Moringa extract was quantified, and biocompatibility was confirmed cell viability, adhesion, and differentiation assays using B65 and PC12 cells. These scaffolds significantly enhanced cell proliferation, neurite outgrowth, and neural differentiation, as demonstrated by DAPI staining, SEM, MTT, qRT-PCR, and immunocytochemistry. , a sciatic nerve transection model in rats was used to assess the regenerative potential of the conduits. After 12 weeks, improvements in sciatic functional index, electrophysiology, muscle recovery, and nerve histology were observed. Immunohistochemistry, qRT-PCR, and TEM further confirmed myelin sheath regeneration and neuromuscular recovery. These findings suggest that NGCs incorporating CQDs and Moringa extract provide a synergistic platform for promoting nerve regeneration and functional recovery, offering a novel and effective approach for the treatment of peripheral nerve injuries (PNI).
神经导向导管（NGCs）是一种很有前景的策略，可在周围神经再生过程中支持轴突生长和定向。聚己内酯（PCL）为NGCs制造提供了合适的机械性能和可生物降解性能。为了增强其功能，碳量子点（CQDs）可以改善物理和化学性质，而提取物含有支持神经发生和神经修复的生物活性化合物。在本研究中，制备并评估了由PCL、CQDs和辣木提取物组成的静电纺丝排列纳米纤维支架。同时含有CQDs和辣木的支架显示出改善的机械强度、亲水性和降解特性。对辣木提取物的释放进行了定量，并通过使用B65和PC12细胞的细胞活力、粘附和分化试验证实了生物相容性。如DAPI染色、扫描电子显微镜、MTT、qRT-PCR和免疫细胞化学所示，这些支架显著增强了细胞增殖、神经突生长和神经分化。此外，使用大鼠坐骨神经横断模型评估导管的再生潜力。12周后，观察到坐骨神经功能指数、电生理学、肌肉恢复和神经组织学方面的改善。免疫组织化学、qRT-PCR和透射电子显微镜进一步证实了髓鞘再生和神经肌肉恢复。这些发现表明，结合CQDs和辣木提取物的NGCs为促进神经再生和功能恢复提供了一个协同平台，为治疗周围神经损伤（PNI）提供了一种新颖有效的方法。
