Belkin V M, Zlatopol'skiĭ A D, Zykova T A, Mazurov V I
Vopr Med Khim. 1984 Mar-Apr;30(2):120-5.
Interaction of 125I-collagen with fibronectin-synthesizing polyribosomes, isolated from 9 days old chicken embryos, was studied. The interaction was maximal at the ratio of 0.1 microgram 125I-collagen and 5 ou of polyribosomes. Specific reaction of 125I-collagen with fibronectin polypeptides growing on polyribosomes was estimated by means of antibodies to fibronectin inhibiting the complex formation of polyribosomes-125I-collagen. The antibodies were shown to decrease the reaction rate of total polyribosomes (free and membrane-bound) with 125I-collagen by 25.4% and of free polyribosomes - by 6.7%. The data obtained suggest that fibronectin is synthesized mainly on polyribosomes, bound with the membranes of endoplasmic reticulum.
研究了从9日龄鸡胚中分离出的125I-胶原蛋白与合成纤连蛋白的多核糖体之间的相互作用。当125I-胶原蛋白与多核糖体的比例为0.1微克和5个单位时，相互作用最大。通过抗纤连蛋白抗体抑制多核糖体-125I-胶原蛋白的复合物形成，来评估125I-胶原蛋白与在多核糖体上生长的纤连蛋白多肽的特异性反应。结果表明，抗体可使总多核糖体（游离的和膜结合的）与125I-胶原蛋白的反应速率降低25.4%，使游离多核糖体的反应速率降低6.7%。所得数据表明，纤连蛋白主要在内质网膜结合的多核糖体上合成。
