Gorbach Z V, Maglysh S S, Kubyshin V L, Ostrovskiĭ Iu M
Biokhimiia. 1985 Aug;50(8):1261-8.
Thiamine deficiency in rats induced by oxythiamine is accompanied by an increase in the free NADP+/NADPH ratio in liver tissue, which results in multifold stimulation of the metabolite flux in the oxidation branch of the pentose cycle. The increase in the intracellular concentrations of isocitrate and alpha-ketoglutarate with a simultaneous decrease of malate in the liver of vitamin-deficient rats points to the inhibition of alpha-ketoglutarate dehydrogenase responsible for the anomalous metabolism under conditions of thiamine deficiency. The decrease of the functional activity of the tricarboxylic acid cycle is concomitant with the activation of conversions in the oxidation branch of the pentose cycle, glucuronate and glycolytic pathways of carbohydrate metabolism, which is directed at eliminating the energy deficiency in rats with B1-hypovitaminosis.
由氧硫胺素诱导的大鼠硫胺素缺乏伴随着肝脏组织中游离NADP⁺/NADPH比值的增加，这导致戊糖循环氧化分支中代谢物通量的多重刺激。维生素缺乏大鼠肝脏中异柠檬酸和α-酮戊二酸细胞内浓度的增加同时苹果酸减少，这表明硫胺素缺乏条件下负责异常代谢的α-酮戊二酸脱氢酶受到抑制。三羧酸循环功能活性的降低与戊糖循环氧化分支、糖醛酸和碳水化合物代谢糖酵解途径中转化的激活同时发生，这旨在消除维生素B1缺乏大鼠的能量不足。
