Gorbach Z V, Maglysh S S, Ostrovskiĭ Iu M
Biokhimiia. 1983 Nov;48(11):1778-82.
It was found that the progressive development of vitamin B1 deficiency in rats caused by varying doses of oxythiamine results in a sharp decrease of the free NAD/NADH ratio in the liver after injection of a high dose of the antivitamin. The value of this ratio was calculated from the intracellular concentrations of pyruvate, lactate and Keq for lactate dehydrogenase, whose activity remained practically unchanged throughout the experiments. The increase of the cAMP level in the liver caused by corresponding doses of oxythiamine was concomitant with a marked activation of cAMP-dependent processes.
结果发现，由不同剂量的氧硫胺素导致的大鼠体内维生素B1缺乏的逐步发展，在注射高剂量抗维生素后，会使肝脏中游离NAD/NADH比值急剧下降。该比值由丙酮酸、乳酸的细胞内浓度以及乳酸脱氢酶的Keq计算得出，在整个实验过程中其活性基本保持不变。相应剂量的氧硫胺素引起的肝脏中cAMP水平升高与cAMP依赖性过程的显著激活同时发生。
