Gorbach Z V, Ostrovskiĭ Iu M, Maglysh S S, Borodinskiĭ A N
Vopr Med Khim. 1980 Sep-Oct;26(5):608-12.
Inhibition of rat liver transketolase under conditions of hydroxythiamin or alimentary B1 avitaminosis was accompanied by an increase in intracellular concentration of 6-phosphogluconate and xylulose-5-phosphate. The alterations in their content was inversely correlated with the transketolase activity. The dehydrogenases involved in the pentose phosphate shunt exhibited various unspecific sensitivity to hydroxythiamin. The doses of the antimetabolites up to 0.4 mg per rat did not alter the activity of the pentose phosphate shunt dehydrogenases; an increase in the dose from 4 up to 20 mg led to an inhibition of the enzymes. Possible biochemical alteration, occurring in response to various degree of transketolase inhibitions, are discussed.
在羟基硫胺或饮食性维生素B1缺乏症的情况下，大鼠肝脏转酮醇酶受到抑制，同时6-磷酸葡萄糖酸和木酮糖-5-磷酸的细胞内浓度增加。它们含量的变化与转酮醇酶活性呈负相关。戊糖磷酸途径中的脱氢酶对羟基硫胺表现出不同的非特异性敏感性。每只大鼠高达0.4毫克的抗代谢物剂量不会改变戊糖磷酸途径脱氢酶的活性；剂量从4毫克增加到20毫克会导致酶的抑制。讨论了因转酮醇酶抑制程度不同而可能发生的生化改变。
