Vasconcelos Mariana, Piao Ying, Himbert Sebastian, Fellner Andreas, Wang Fengyan, Liu Jun, Fefer Michael, Shen Youqing, Rheinstädter Maikel C, Sundin George W, Plaetzer Kristjan
Laboratory of Photodynamic Inactivation of Microorganisms, Department of Biosciences and Medical Biology, University of Salzburg, Hellbrunner Str. 34, Salzburg 5020, Austria.
Zhejiang Key Laboratory of Smart BioMaterials and Center for Bionanoengineering, College of Chemical and Biological Engineering, Zhejiang University, Hangzhou 310027, China.
J Agric Food Chem. 2025 Aug 13;73(32):19958-19973. doi: 10.1021/acs.jafc.5c02074. Epub 2025 Aug 1.
Fire blight, caused by , severely affects apple and pear orchards on a global scale. Conventional treatment includes the application of antibiotics such as streptomycin during bloom, which promotes resistance. Photodynamic inactivation (PDI) is based on the light-induced and photosensitizer-mediated overproduction of reactive oxygen species for killing microbes. This study develops PDI using the natural photosensitizer sodium magnesium chlorophyllin (Chl) from the laboratory to field application. Laboratory experiments showed commercial-ready SUN-D products (Chl + EDTA) were highly effective against (395 nm LED, total kill at 53.2 J cm), regardless of streptomycin resistance phenotype. No resistance to Chl developed after 15 treatment cycles. Molecular dynamics simulations suggest that EDTA lowers the energy barrier for chlorin e6, enhancing membrane penetration. Field trials at two locations using sunlight illumination showed that SUN-D controlled blossom blight comparably to antibiotics. Photodynamic inactivation with SUN-D offers a promising, resistance-independent addition to current fire blight management strategies.
火疫病由[病原体名称缺失]引起，在全球范围内严重影响苹果园和梨园。传统治疗方法包括在花期施用抗生素如链霉素，但这会促进抗药性。光动力灭活（PDI）基于光诱导和光敏剂介导的活性氧过量产生来杀灭微生物。本研究将实验室中使用天然光敏剂叶绿酸镁钠（Chl）的光动力灭活技术从实验室应用拓展到田间应用。实验室实验表明，商业化的SUN-D产品（Chl + EDTA）对[病原体名称缺失]高效（395 nm LED，在53.2 J/cm²时完全杀灭），无论链霉素抗性表型如何。经过15个处理周期后未产生对Chl的抗性。分子动力学模拟表明，EDTA降低了二氢卟吩e6的能垒，增强了其对细胞膜的穿透能力。在两个地点进行的利用阳光照射的田间试验表明，SUN-D对花腐病的防治效果与抗生素相当。使用SUN-D进行光动力灭活为当前火疫病管理策略提供了一种有前景的、不依赖抗性的补充方法。
