不同物种棕色脂肪组织解偶联蛋白及其前体的大小分析。
Size analysis of uncoupling protein and its precursor from brown adipose tissue of different species.
作者信息
Freeman K B, Meyrick K, Patel H V, Ridley R G
出版信息
Can J Biochem Cell Biol. 1985 Sep;63(9):988-91. doi: 10.1139/o85-122.
The apparent size of the monomeric form of uncoupling protein from brown adipose tissue of several mammalian species was compared by sodium dodecyl sulfate - polyacrylamide gel electrophoresis. Including earlier results the apparent molecular mass of the protein from rat was about 32 000 daltons and varied about 1500-2000 daltons from the different sources with the size increasing in the order human less than rat less than or equal to mouse less than hamster less than or equal to rabbit. The size of newly synthesized uncoupling protein was also found to vary among species. However in the two cases examined, rat and rabbit, the precursor and its respective mature monomeric protein had the same apparent size as shown by coelectrophoresis.
通过十二烷基硫酸钠-聚丙烯酰胺凝胶电泳比较了几种哺乳动物棕色脂肪组织中解偶联蛋白单体形式的表观大小。结合早期结果，大鼠来源的该蛋白表观分子量约为32000道尔顿，不同来源的分子量相差约1500 - 2000道尔顿，其大小顺序为人类小于大鼠小于或等于小鼠小于仓鼠小于或等于兔子。新合成的解偶联蛋白大小在不同物种间也存在差异。然而，在所检测的大鼠和兔子这两种情况下，前体及其各自的成熟单体蛋白经共电泳显示具有相同的表观大小。
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