Fraser J R, Alcorn D, Laurent T C, Robinson A D, Ryan G B
Cell Tissue Res. 1985;242(3):505-10. doi: 10.1007/BF00225415.
The uptake of [3H]acetyl-labelled hyaluronic acid (HA) was examined in the liver, spleen and kidney of the rat after i.v. injection. 3H-activity was located by light- and electron-microscopic autoradiography after measurement by scintillation counting of tissue digests. In the liver, approximately 90% of the radioactivity was located in the sinusoidal endothelial cells, with autoradiographic grains distributed throughout the cytoplasm; 50% of the grains overlay vacuoles 0.3 to 1.2 micron in diameter. A few grains (4%) were located in Disse's space or nearby in the cytoplasm of hepatocytes. No grains were found in Kupffer cells. The remainder were randomly scattered across the sections in a pattern indicating nonspecific background activity. These observations are in accordance with the selective uptake of HA exhibited by dissociated liver cells in vitro. HA concentrations in the spleen and kidney were too low for detection by autoradiography. Splenic concentrations were much lower than in rabbits or mice; in this respect the uptake of circulating HA in the rat resembles that reported for chondroitin 4-sulphate.
静脉注射后，在大鼠的肝脏、脾脏和肾脏中检测了[3H]乙酰标记的透明质酸（HA）的摄取情况。通过对组织消化物进行闪烁计数测量后，用光镜和电镜放射自显影法定位3H活性。在肝脏中，约90%的放射性位于肝血窦内皮细胞，放射自显影颗粒分布于整个细胞质；50%的颗粒覆盖直径为0.3至1.2微米的液泡。少数颗粒（4%）位于狄氏间隙或肝细胞细胞质附近。在枯否细胞中未发现颗粒。其余颗粒以表明非特异性背景活性的模式随机散布在切片中。这些观察结果与离体分离的肝细胞对HA的选择性摄取一致。脾脏和肾脏中的HA浓度过低，无法通过放射自显影检测到。脾脏中的浓度远低于兔子或小鼠；在这方面，大鼠对循环HA的摄取类似于硫酸软骨素4的报道情况。
