Yavorsky Marisol Solange, Norero Natalia Sigrid, Spetter Maximiliano Joaquín, Pereyra Susana Beatriz, Verna Andrea Elizabeth, Luna Naiara Urrutia, Ríos Glenda Laura, González Altamiranda Erika Analía
Laboratorio de Virología Veterinaria, Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible, Instituto Nacional de Tecnología Agropecuaria (INTA) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Balcarce, Argentina; Laboratorio Biotecnología de la Reproducción, Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible, Instituto Nacional de Tecnología Agropecuaria (INTA) - Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Balcarce, Argentina.
Laboratorio de Agrobiotecnología Agrícola, Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible, Instituto Nacional de Tecnología Agropecuaria (INTA)-Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Balcarce, Argentina.
Theriogenology. 2025 Dec;248:117613. doi: 10.1016/j.theriogenology.2025.117613. Epub 2025 Jul 31.
Bovine Viral Diarrhea Virus (BVDV) and anti-BVDV antibodies have been detected in ovarian follicular fluid of infected cows. The aim of this study was to assess both the frequency of BVDV detection and the prevalence of anti-BVDV antibodies in pooled follicular fluid obtained from slaughterhouse ovaries for in vitro embryo production. Additionally, we sought to determine whether specific antibodies present in these samples are able to neutralize different BVDV strains. BVDV RNA was detected by using reverse transcription polymerase chain reaction (RT-PCR) and reverse transcription-quantitative polymerase chain reaction (RT-qPCR), while viral viability was evaluated by using viral isolation (VI). Antibody titers against different BVDV strains (reference strain NADL-1a Vs145; field strain 13/558-1a; field strains 10/49-1b) were determined through viral neutralization (VN) assays. A total of 60 pooled follicular fluid samples from slaughterhouse ovaries were analyzed over a period of 19 months. Results showed that 45 % (27 out of 60) of the samples tested positive for BVDV RNA via RT-PCR and RT-qPCR, although no samples yielded viable virus through the VI technique. The seroprevalence of anti-BVDV antibodies was 98 % (59 out of 60), with antibody titers sufficient (1:136 and 1:268) to neutralize the field strains tested. These findings indicate a high prevalence of BVDV RNA and extensive seroprevalence of anti-BVDV antibodies in pooled follicular fluid samples. The high antibody levels may compromise the sensitivity of VI, likely due to a viral inhibitory effect. Such inhibition could also influence the dynamics of infection throughout the in vitro embryo production process.
在受感染奶牛的卵泡液中已检测到牛病毒性腹泻病毒（BVDV）和抗BVDV抗体。本研究的目的是评估从屠宰场卵巢获取的用于体外胚胎生产的混合卵泡液中BVDV的检测频率和抗BVDV抗体的流行率。此外，我们试图确定这些样本中存在的特异性抗体是否能够中和不同的BVDV毒株。通过逆转录聚合酶链反应（RT-PCR）和逆转录定量聚合酶链反应（RT-qPCR）检测BVDV RNA，同时使用病毒分离（VI）评估病毒活力。通过病毒中和（VN）试验测定针对不同BVDV毒株（参考毒株NADL-1a Vs145；田间毒株13/558-1a；田间毒株10/49-1b）的抗体滴度。在19个月的时间里，共分析了60份来自屠宰场卵巢的混合卵泡液样本。结果显示，通过RT-PCR和RT-qPCR检测，45%（60份样本中的27份）的样本BVDV RNA呈阳性，尽管通过VI技术没有样本产生活病毒。抗BVDV抗体的血清阳性率为98%（60份样本中的59份），抗体滴度足以（1:136和1:268）中和所检测的田间毒株。这些发现表明，在混合卵泡液样本中BVDV RNA的流行率很高，抗BVDV抗体的血清流行率也很广泛。高抗体水平可能会损害VI的敏感性，这可能是由于病毒抑制作用。这种抑制也可能影响整个体外胚胎生产过程中的感染动态。
