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诱导多能干细胞培养基对诱导心脏组织分化潜能的影响。

Effect of iPS cell culture medium on the differentiation potential of induced cardiac tissues.

作者信息

Nakashima Yoshiki, Tsukahara Masayoshi

机构信息

Research and Development Center, CiRA Foundation, Nakanoshima Qross, Osaka, 530-005, Japan.

出版信息

Sci Rep. 2025 Aug 3;15(1):28301. doi: 10.1038/s41598-025-13259-x.

DOI:10.1038/s41598-025-13259-x
PMID:40754549
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12319073/
Abstract

The present study focused on the culture medium of induced pluripotent stem cells (iPSCs) prior to the use of cardiomyocytes differentiation induction medium (pre-culture medium). Seven types (Nos. 1-7) of StemFit AK03 medium (Ajinomoto) for clinical iPSCs with varying compositions were prepared as pre-culture medium. The cardiac muscle troponin T (cTnT) positivity of No. 1 (StemFit AK03 medium) was 84%, No. 3 (similar to E8 medium) was 89%, No. 2 (similar to E8 medium) was 91%, No. 5 (similar to EB Formation medium) was 95%, when using differentiation induction medium prepared with known components available for clinical cell production. The formation of cardiac tissues was assessed by evaluating the expression levels of specific markers, including cTnT, atrial natriuretic peptides (ANP), and pro-B-type natriuretic peptide (proBNP). The results demonstrated that cardiac tissue with high protein expression levels of cTnT and ANP was formed when similar to E8 medium as pre-culture medium.

摘要

本研究聚焦于诱导多能干细胞（iPSCs）在使用心肌细胞分化诱导培养基（预培养基）之前的培养基。制备了七种类型（编号1 - 7）成分各异的用于临床iPSCs的StemFit AK03培养基（味之素公司）作为预培养基。当使用由临床细胞生产可用的已知成分制备的分化诱导培养基时，1号（StemFit AK03培养基）的心肌肌钙蛋白T（cTnT）阳性率为84%，3号（类似于E8培养基）为89%，2号（类似于E8培养基）为91%，5号（类似于EB形成培养基）为95%。通过评估包括cTnT、心钠素（ANP）和B型利钠肽原（proBNP）在内的特定标志物的表达水平来评估心脏组织的形成。结果表明，当使用类似于E8培养基作为预培养基时，形成了cTnT和ANP蛋白表达水平较高的心脏组织。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171d/12319073/a1595688de83/41598_2025_13259_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171d/12319073/8372d3ba1b82/41598_2025_13259_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171d/12319073/8bcc864824f2/41598_2025_13259_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171d/12319073/74599e5092b3/41598_2025_13259_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171d/12319073/e087a8e3222e/41598_2025_13259_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171d/12319073/ac79576a73f6/41598_2025_13259_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171d/12319073/a1595688de83/41598_2025_13259_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171d/12319073/8372d3ba1b82/41598_2025_13259_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171d/12319073/8bcc864824f2/41598_2025_13259_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171d/12319073/74599e5092b3/41598_2025_13259_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171d/12319073/e087a8e3222e/41598_2025_13259_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171d/12319073/ac79576a73f6/41598_2025_13259_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/171d/12319073/a1595688de83/41598_2025_13259_Fig6_HTML.jpg

相似文献

[1]
Effect of iPS cell culture medium on the differentiation potential of induced cardiac tissues.

Sci Rep. 2025-8-3

[2]
Natriuretic peptides modulate monocyte-derived Langerhans cell differentiation and promote a migratory phenotype.

Front Immunol. 2025-6-9

[3]
DMSO-free cryopreservation of hiPSC-derived cardiomyocytes: low temperature characterization and protocol development.

Stem Cell Res Ther. 2025-6-10

[4]
Neprilysin 2 catalyses the degradation of natriuretic peptides despite sacubitrilat Inhibition.

Sci Rep. 2025-7-28

[5]
Protein-free media for cardiac differentiation of hPSCs in 2000 mL suspension culture.

Stem Cell Res Ther. 2024-7-18

[6]
A systematic review of the diagnostic accuracy of natriuretic peptides for heart failure.

Arch Intern Med. 2004-10-11

[7]
Retinoic acid modulation guides human-induced pluripotent stem cell differentiation towards left or right ventricle-like cardiomyocytes.

Stem Cell Res Ther. 2024-6-21

[8]
Addressing bioreactor hiPSC aggregate stability, maintenance and scaleup challenges using a design of experiment approach.

Stem Cell Res Ther. 2024-7-2

[9]
Associations between Growth Differentiation Factor 15, Cardiac Troponin T, and N-terminal pro-B-type Natriuretic Peptide, and Future Myocardial Fibrosis Assessed by Cardiac Magnetic Resonance Imaging: Data from the Akershus Cardiac Examination 1950 Study.

J Appl Lab Med. 2025-3-3

[10]
Brain natriuretic peptide and N-terminal brain natriuretic peptide for the diagnosis of haemodynamically significant patent ductus arteriosus in preterm neonates.

Cochrane Database Syst Rev. 2022-12-8

本文引用的文献

[1]
Organoids from pluripotent stem cells and human tissues: When two cultures meet each other.

Dev Cell. 2025-2-24

[2]
Engineering the 3D structure of organoids.

Stem Cell Reports. 2025-1-14

[3]
Harnessing the regenerative potential of interleukin11 to enhance heart repair.

Nat Commun. 2024-11-8

[4]
Atelocollagen supports three-dimensional culture of human induced pluripotent stem cells.

Mol Ther Methods Clin Dev. 2024-7-20

[5]
The MEK-ERK signaling pathway promotes maintenance of cardiac chamber identity.

Development. 2024-2-15

[6]
Simultaneous binding of bFGF to both FGFR and integrin maintains properties of primed human induced pluripotent stem cells.

Regen Ther. 2023-12-23

[7]
Improved Production of Induced Pluripotent Stem Cells Using Dot Pattern Culture Plates.

Tissue Eng Part C Methods. 2023-9

[8]
Nutritional requirements of human induced pluripotent stem cells.

Stem Cell Reports. 2023-6-13

[9]
Semi-3D cultures using Laminin 221 as a coating material for human induced pluripotent stem cells.

Regen Biomater. 2022-9-5

[10]
A review of protocols for human iPSC culture, cardiac differentiation, subtype-specification, maturation, and direct reprogramming.

STAR Protoc. 2022-9-16

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