Nakashima Yoshiki, Tsukahara Masayoshi
Research and Development Center, CiRA Foundation, Nakanoshima Qross, Osaka, 530-005, Japan.
Sci Rep. 2025 Aug 3;15(1):28301. doi: 10.1038/s41598-025-13259-x.
The present study focused on the culture medium of induced pluripotent stem cells (iPSCs) prior to the use of cardiomyocytes differentiation induction medium (pre-culture medium). Seven types (Nos. 1-7) of StemFit AK03 medium (Ajinomoto) for clinical iPSCs with varying compositions were prepared as pre-culture medium. The cardiac muscle troponin T (cTnT) positivity of No. 1 (StemFit AK03 medium) was 84%, No. 3 (similar to E8 medium) was 89%, No. 2 (similar to E8 medium) was 91%, No. 5 (similar to EB Formation medium) was 95%, when using differentiation induction medium prepared with known components available for clinical cell production. The formation of cardiac tissues was assessed by evaluating the expression levels of specific markers, including cTnT, atrial natriuretic peptides (ANP), and pro-B-type natriuretic peptide (proBNP). The results demonstrated that cardiac tissue with high protein expression levels of cTnT and ANP was formed when similar to E8 medium as pre-culture medium.
本研究聚焦于诱导多能干细胞（iPSCs）在使用心肌细胞分化诱导培养基（预培养基）之前的培养基。制备了七种类型（编号1 - 7）成分各异的用于临床iPSCs的StemFit AK03培养基（味之素公司）作为预培养基。当使用由临床细胞生产可用的已知成分制备的分化诱导培养基时，1号（StemFit AK03培养基）的心肌肌钙蛋白T（cTnT）阳性率为84%，3号（类似于E8培养基）为89%，2号（类似于E8培养基）为91%，5号（类似于EB形成培养基）为95%。通过评估包括cTnT、心钠素（ANP）和B型利钠肽原（proBNP）在内的特定标志物的表达水平来评估心脏组织的形成。结果表明，当使用类似于E8培养基作为预培养基时，形成了cTnT和ANP蛋白表达水平较高的心脏组织。
