Saffhill R, Hall J A
Chem Biol Interact. 1985 Dec 31;56(2-3):363-70. doi: 10.1016/0009-2797(85)90017-1.
O4-MedTMP and O6-MedGMP may be incorporated from their 5'-triphosphates into polynucleotides by DNA polymerases. Once incorporated O6-MedGMP leads to errors during further cycles of DNA synthesis with a mutagenic efficiency of 0.3-0.7, the value depending upon the relative dNTP concentrations in the assay. In contrast O4-MedTMP leads to very few errors following its incorporation into DNA. The results demonstrate that O6-MedGMP, once incorporated into DNA, should be destined to give rise to DNA base changes during the second round of DNA synthesis.
O4-甲基胸苷三磷酸（O4-MedTMP）和O6-甲基鸟苷三磷酸（O6-MedGMP）可被DNA聚合酶从其5'-三磷酸形式掺入多核苷酸中。一旦掺入，O6-MedGMP在随后的DNA合成循环中会导致错误，诱变效率为0.3-0.7，该值取决于测定中相对的脱氧核苷酸三磷酸（dNTP）浓度。相比之下，O4-MedTMP掺入DNA后导致的错误极少。结果表明，O6-MedGMP一旦掺入DNA，在第二轮DNA合成过程中应会导致DNA碱基变化。
