DNA聚合酶I和α将O6-甲基脱氧鸟苷单磷酸和O4-甲基脱氧胸苷单磷酸掺入DNA中。
The incorporation of O6-methyldeoxyguanosine and O4-methyldeoxythymidine monophosphates into DNA by DNA polymerases I and alpha.
作者信息
Hall J A, Saffhill R
出版信息
Nucleic Acids Res. 1983 Jun 25;11(12):4185-93. doi: 10.1093/nar/11.12.4185.
Abstract
The modified nucleoside 5'-triphosphates O6-MedGTP ad O4-MedTTP have been synthesised and their acceptability as DNA-precursors investigated using DNA polymerases I and alpha in an in vitro assay. O6-MedGMP is only incorporated into newly-synthesized DNA-like material in the presence of templates containing thymine bases. Similarly O4-MedTMP is only incorporated in the presence of templates containing guanine bases. The results confirm the promutagenic nature and base-pairing properties of O6-MeG ad O4-MeT.
摘要
已合成了修饰核苷5'-三磷酸O6-甲基鸟苷三磷酸（O6-MedGTP）和O4-甲基胸苷三磷酸（O4-MedTTP），并在体外试验中使用DNA聚合酶I和α研究了它们作为DNA前体的可接受性。只有在含有胸腺嘧啶碱基的模板存在下，O6-甲基鸟苷一磷酸（O6-MedGMP）才会掺入新合成的类DNA物质中。同样，只有在含有鸟嘌呤碱基的模板存在下，O4-甲基胸苷一磷酸（O4-MedTMP）才会掺入。结果证实了O6-甲基鸟嘌呤（O6-MeG）和O4-甲基胸腺嘧啶（O4-MeT）的促诱变性质和碱基配对特性。
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